Examination of the oral mucosa and peripheral blood cells of patients with recurrent aphthous ulceration for human herpesvirus DNA
| Autor: | William L. Weston, Dennis Cook, Sylvia L. Brice, Maureen Leahy, J.Clark Huff |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty viruses medicine.disease_cause Polymerase Chain Reaction Peripheral blood mononuclear cell Herpesviridae Virus Lesion Alphaherpesvirinae medicine Humans Prospective Studies Viremia Oral mucosa General Dentistry Chi-Square Distribution biology business.industry Mouth Mucosa Varicella zoster virus biology.organism_classification Herpes simplex virus medicine.anatomical_structure Otorhinolaryngology Case-Control Studies DNA Viral Leukocytes Mononuclear Female Stomatitis Aphthous Surgery Oral Surgery medicine.symptom business |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 89:193-198 |
| ISSN: | 1079-2104 |
| Popis: | Objective. The purpose of this study was to exam the oral mucosa and peripheral blood cells of patients with recurrent aph-thous ulceration (RAU) for the presence of the following human herpesviruses: herpes simplex viruses 1 and 2, varicella zoster virus, Epstein-Barr virus, cytomegalovirus, human herpesvirus-6, and human herpesvirus-7. Study design. Fifty-eight subjects with RAU and 10 control subjects were recruited at an academic referral center and enrolled in this prospective, nonrandomized, case-controlled study. Each of the subjects with RAU was seen during an acute episode, and swab specimens from lesional (RAU-acute/lesion) and clinically normal (RAU-acute/normal) oral mucosa were obtained. Each of 2 subjects with RAU was evaluated during more than one acute episode. Three subjects with RAU were seen between active episodes, and swab specimens were taken from clinically normal (RAU-convalescent) oral mucosa. Swab specimens from clinically normal (control/normal) oral mucosa were obtained from the control subjects. Peripheral blood specimens were obtained from subjects with RAU and control subjects at the time the swab specimens were performed. Through use of polymerase chain reaction, all swab and peripheral blood specimens were examined for the presence of human herpesvirus DNA. Statistical significance was determined by means of χ 2 analysis. Results. Herpes simplex virus and human herpesvirus-6 were found in a higher percentage of mucosal specimens from the control subjects (herpes simplex virus, 4/10; human herpesvirus-6, 5/9) than from the subjects with RAU (RAU-acute/lesion: 3/45 herpes simplex virus, 13/53 human herpesvirus-6; RAU-acute/normal: 7/48 herpes simplex virus, 9/53 human herpesvirus-6). No difference was demonstrated between RAU-acute/lesion, RAU-acute/normal, and RAU-convalescent mucosal specimens for any of the human herpesviruses. Different human herpesviruses were identified from individual subjects with RAU during subsequent episodes of disease. Epstein-Barr virus (6/35), human herpesvirus-6 (3/40), and human herpesvirus-7 (7/43) were detected in the peripheral blood mononuclear cells during acute RAU but not in RAU-convalescent or control peripheral blood mononuclear cells. Conclusions. The detection of human herpesvirus DNA from the oral mucosa and peripheral blood mononuclear cells of patients with RAU appears to represent normal viral shedding rather than a direct causal mechanism in this disorder. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2000;89:193-8) |
| Databáze: | OpenAIRE |
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