Utility of Alternate, Noncentromeric Chromosome 17 Reference Probe for Human Epidermal Growth Factor Receptor Fluorescence In Situ Hybridization Testing in Breast Cancer Cases
| Autor: | Asawari Patil, Trupti Pai, Omshree Shetty, Angad Singh, Tanuja Shet, Sangeeta B. Desai |
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| Rok vydání: | 2018 |
| Předmět: |
Monosomy
Pathology medicine.medical_specialty Receptor ErbB-2 Breast Neoplasms Locus (genetics) In situ hybridization Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Breast cancer Biomarkers Tumor medicine Humans 030212 general & internal medicine In Situ Hybridization Fluorescence Polysomy medicine.diagnostic_test Hybridization probe General Medicine medicine.disease Chromosome 17 (human) Medical Laboratory Technology 030220 oncology & carcinogenesis Female Chromosomes Human Pair 17 Fluorescence in situ hybridization |
| Zdroj: | Archives of Pathology & Laboratory Medicine. 142:626-633 |
| ISSN: | 1543-2165 0003-9985 |
| Popis: | Context PathVysion—a US Food and Drug Administration–approved dual-probe human epidermal growth factor receptor (HER2) fluorescence in situ hybridization (FISH) assay—provides the HER2:CEP17 ratio, a centromeric enumeration probe ratio for determining HER2 status in breast cancers. However, pericentromeric amplifications might then skew the HER2:CEP17 ratio, underestimating the HER2 status, which calls into question the use of CEP17 as the reference probe. Objective To analyze the utility of a noncentromeric chromosome 17 reference locus (D17S122) to assess HER2 gene status in cases showing “nonclassical” FISH patterns with the CEP17 probe. Design The HER2 status of breast cancers accessioned in the years 2015–2017, displaying “nonclassical” or “equivocal” results by the PathVysion (Abbott Molecular Inc, Des Plaines, Illinois) HER2 DNA Probe Kit were reflex tested using an alternate FISH probe (ZytoLight SPEC/D17S122, ZytoVision, Bremerhaven, Germany) and interpreted with American Society of Clinical Oncology/College of American Pathologists 2013 guidelines. Results Of 37 cases, 17 were FISH equivocal. With the alternate D17S122 probe, 13 (76.4%) were reclassified as amplified, 3 (17.6%) as nonamplified, and a single case retained an equivocal result. Of the 17 cases with a chromosome 17 polysomy pattern, disomy, polysomy, and monosomy patterns were seen with 14 cases, 2 cases, and 1 case, respectively. Within the 17 cases with polysomy pattern, 3 (17.6%) demonstrated an unusual colocalization pattern of HER2 and CEP17, which was not observed with the alternate probe. Conclusions The denominator-stable alternate probe is a useful adjunct in the diagnostic armamentarium to analyze HER2 status in cases with FISH equivocal and complex patterns. |
| Databáze: | OpenAIRE |
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