Incidence and Relative Prevalence of Distinct Caulimoviruses (Genus Caulimovirus, Family Caulimoviridae) Associated with Dahlia Mosaic in Dahlia variabilis

Autor:	K. B. Druffel, Hanu R. Pappu, R. Miglino, A. Jodlowska, A. R. van Schadewijk, V. Pahalawatta
Rok vydání:	2007
Předmět:	Dahlia Caulimovirus ved/biology ved/biology.organism_classification_rank.species Plant Science Biology biology.organism_classification Dahlia pinnata Virology Genome Virus law.invention law Microbial genetics Caulimoviridae Agronomy and Crop Science Polymerase chain reaction
Zdroj:	Plant Disease. 91:1194-1197
ISSN:	1943-7692 0191-2917
DOI:	10.1094/pdis-91-9-1194
Popis:	Dahlia mosaic, caused by Dahlia mosaic virus (DMV), is one of the most important viral diseases of dahlia. Molecular characterization of DMV showed the association of two distinct caulimoviruses (DMV-D10, DMV-Portland) and a D10-like sequence variant (DMV-Holland) with the disease. Using primers specific to these two viruses and the sequence variant, a polymerase chain reaction–based assay was used to determine their relative incidence in several dahlia samples from the United States and the Netherlands. Testing was done on samples collected in 2005 and 2006 in the United States and in 2006 in the Netherlands. Results indicated the predominance of DMV-D10 over DMV-Portland and DMV-Holland in both the United States and the Netherlands. Using conserved regions of the viral genome, primers were designed and used to detect all three sequences. Results suggested that DMV-D10 is predominantly associated with dahlia mosaic, but diagnostics should also include testing for DMV-Portland and DMV-Holland.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1d651a3d1502918b6afcee6e91d4509 https://doi.org/10.1094/pdis-91-9-1194 Zobrazit plný text záznamu