Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis
| Autor: | Sabaheta Bektas, Damir Rebić, Mufida Aljicevic, Ana Budimir, Velma Rebic, Sabina Mahmutovic Vranic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CA-MRSA Fusidic acid 030106 microbiology Population markers Biology medicine.disease_cause antimicrobial susceptibility Microbiology 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance medicine Pulsed-field gel electrophoresis education Original Paper education.field_of_study SCCmec Clindamycin General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Virology infection chemistry Linezolid medicine.drug |
| Zdroj: | Acta Informatica Medica |
| DOI: | 10.5455/aim.2016.24.248-252 |
| Popis: | BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to analyze MRSA strains from the general population in Canton Sarajevo, B&H. ----- METHODS: Our investigation including either phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and Panton-Valentine leukocidin (PVL) detection. ----- RESULTS: Antimicrobial susceptibility: all MRSA isolates were resistant to the β-lactam antibiotics tested, and all isolates were susceptible trimethoprim sulphamethoxazole, rifampicin, fusidic acid, linezolid and vancomycin. Sixty-eight per cent of the MRSA isolates were resistant to erythromycin, 5% to clindamycin, 5% to gentamicin and 4% to ciprofloxacin. After the PFGE analysis, the isolates were grouped into five similarity groups: A-E. The largest number of isolates belonged to one of two groups: C: 60 (60%) and D: 27 (27%). In both groups C and D, SCCmec type IV was predominant (60% and 88, 8%, respectively). A total of 24% of the isolates had positive expression of PVL genes, while 76% showed a statistically significantly greater negative expression of PVL genes. ----- CONCLUSION: SCCmec type IV, together with the susceptibility profile and PFGE grouping, is considered to be typical of CA-MRSA. |
| Databáze: | OpenAIRE |
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