AS1411 Aptamer-Anionic Linear Globular Dendrimer G2-Iohexol Selective Nano-Theranostics
| Autor: | Pardis Mohammadzadeh, Mehdi Shafiee Ardestani, Seyedeh Masoumeh Ghoreishi, Reza Ahangari Cohan, Ahmad Bitarafan-Rajabi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Dendrimers Pathology medicine.medical_specialty Iohexol Aptamer lcsh:Medicine Breast Neoplasms 02 engineering and technology Conjugated system Article Theranostic Nanomedicine 03 medical and health sciences Drug Delivery Systems In vivo Dendrimer medicine Humans lcsh:Science Multidisciplinary Chemistry lcsh:R RNA-Binding Proteins Phosphoproteins 021001 nanoscience & nanotechnology In vitro Neoplasm Proteins HEK293 Cells 030104 developmental biology Cancer cell MCF-7 Cells Biophysics lcsh:Q Female 0210 nano-technology Nucleolin medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-16 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-12150-8 |
| Popis: | Molecular theranostics is of the utmost interest for diagnosis as well as treatment of different malignancies. In the present study, anionic linear globular dendrimer G2 is employed as a suitable carrier for delivery and AS1411 aptamer is exploited as the targeting agent to carry Iohexol specifically to the human breast cancer cells (MCF-7). Dendrimer G2 was prepared and conjugation of dendrimer and aptamer was carried out thereafter. Based on the data yielded by AFM, morphology of smooth and spherical non-targeted dendrimer changed to the rough aspherical shape when it conjugated. Then, conjugation was confirmed using DLS, ELS and SLS methods. Toxicity on nucleolin positive MCF-7 cells and nucleolin negative HEK-293 cells was assessed by XTT and apoptosis/necrosis assays. In vitro uptake was determined using DAPI-FITC staining and ICP-MS methods. In vivo studies including in vivo CT imaging, pathology and blood tests were done to confirm the imaging ability, bio-safety and targeted nature of the Nano-Theranostics in vivo. In a nutshell, the prepared construction showed promising effects upon decreasing the toxicity of Iohexol on normal cells and accumulation of it in the cancer tumors as well as reducing the number of cancer cells. |
| Databáze: | OpenAIRE |
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