Structural and Functional Effects of Cardiomyopathy-Causing Mutations in the Troponin T-Binding Region of Cardiac Tropomyosin
| Autor: | Galina V. Kopylova, Alexander M. Matyushenko, Anastasia V. Pivovarova, Katerina E. Popruga, Sergey Y. Bershitsky, Dmitrii I. Levitsky, Natalya V. Artemova, D. V. Shchepkin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gene isoform Troponin T binding Cardiomyopathy Mutation Missense macromolecular substances Tropomyosin Calorimetry Biochemistry 03 medical and health sciences Protein Domains Troponin T medicine Missense mutation Humans Genetic Predisposition to Disease Protein Unfolding Genetics Binding Sites biology Protein Stability Circular Dichroism Myocardium Cardiac muscle Hypertrophic cardiomyopathy Temperature Cardiomyopathy Hypertrophic medicine.disease Troponin Actins Cell biology Actin Cytoskeleton 030104 developmental biology medicine.anatomical_structure biology.protein Calcium Protein Binding |
| Zdroj: | Biochemistry. 56(1) |
| ISSN: | 1520-4995 |
| Popis: | Hypertrophic cardiomyopathy (HCM) is a severe heart disease caused by missense mutations in genes encoding sarcomeric proteins of cardiac muscle. Many of these mutations are identified in the gene encoding the cardiac isoform of tropomyosin (Tpm), an α-helical coiled-coil actin-binding protein that plays a key role in Ca2+-regulated contraction of cardiac muscle. We employed various methods to characterize structural and functional features of recombinant human Tpm species carrying HCM mutations that lie either within the troponin T-binding region in the C-terminal part of Tpm (E180G, E180V, and L185R) or near this region (I172T). The results of our structural studies show that all these mutations affect, although differently, the thermal stability of the C-terminal part of the Tpm molecule: mutations E180G and I172T destabilize this part of the molecule, whereas mutation E180V strongly stabilizes it. Moreover, various HCM-causing mutations have different and even opposite effects on the stability of the ... |
| Databáze: | OpenAIRE |
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