Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients
| Autor: | Péter Gaszner, Maria Sasvari-Szekely, Zsolt Ronai, András Guttman, Krisztina Boór, Zsofia Nemoda, Huba Kalász |
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| Rok vydání: | 2002 |
| Předmět: |
Genotype
Substance-Related Disorders Buccal swab Biology Biochemistry law.invention chemistry.chemical_compound law Drug Discovery Dopamine receptor D4 Humans Nanotechnology Allele Genotyping Gene Polymerase chain reaction DNA Primers Pharmacology Base Sequence Receptors Dopamine D2 Organic Chemistry Receptors Dopamine D4 Molecular biology chemistry biology.protein Molecular Medicine DNA |
| Zdroj: | Current medicinal chemistry. 9(8) |
| ISSN: | 0929-8673 |
| Popis: | A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele. |
| Databáze: | OpenAIRE |
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