Electrochemical determination of lead and glutathione in a plant cell culture
| Autor: | Bořivoj Klejdus, Jiří Petřek, Jan Vacek, František Jelen, Ladislav Havel, René Kizek, Libuše Trnková |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Inorganic chemistry
Cell Culture Techniques Biophysics chemistry.chemical_element Ethylenediaminetetraacetic acid 02 engineering and technology 01 natural sciences chemistry.chemical_compound Electrochemistry Chelation Sulfhydryl Compounds Picea Physical and Theoretical Chemistry Voltammetry Cells Cultured Detection limit 010401 analytical chemistry General Medicine Hydrogen-Ion Concentration Dropping mercury electrode 021001 nanoscience & nanotechnology Glutathione 0104 chemical sciences Anodic stripping voltammetry Lead chemistry Cell culture 0210 nano-technology Cobalt Nuclear chemistry |
| Zdroj: | Bioelectrochemistry. 63:347-351 |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2003.08.007 |
| Popis: | In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture. |
| Databáze: | OpenAIRE |
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