Electro-acupuncture Suppresses AXL Expression in Dorsal Root Ganglion Neurons and Enhances Analgesic Effect of AXL Inhibitor in Spinal Nerve Ligation Induced-Neuropathic Pain Rats
| Autor: | Xiao-Cui Yuan, Hong Jia, Linping Xu, Jun Zhang, Lingli Liang, Shuyang Chang, Siqi Wei, Yue Dong |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Neurology Analgesic Pharmacology Biochemistry Receptor tyrosine kinase Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Dorsal root ganglion Ganglia Spinal medicine Animals Ligation Analgesics Sulfonamides biology business.industry Receptor Protein-Tyrosine Kinases General Medicine Peripheral Blot Electroacupuncture Pyrimidines Spinal Nerves 030104 developmental biology medicine.anatomical_structure Hyperalgesia Neuropathic pain biology.protein Neuralgia Phosphorylation business 030217 neurology & neurosurgery |
| Zdroj: | Neurochemical Research. 46:504-512 |
| ISSN: | 1573-6903 0364-3190 |
| Popis: | Electro-acupuncture (EA) has been used for clinic analgesia for many years. However, its mechanisms are not fully understood. We recently reported that AXL, a tyrosine kinase receptor, contributes to the peripheral mechanism of neuropathic pain. We here aim to figure out the significance of EA on neuropathic pain mediated by AXL in dorsal root ganglion (DRG). Spinal nerve ligation (SNL) was used as a neuropathic pain model. EA was applied at ''Huantiao'' (GB-30) and ''Yanglingquan'' (GB-34) acupoints for 30 min daily from day 7 to day 10 after SNL. EA not only gradually attenuated SNL-induced mechanical allodynia, but also suppressed the expression of phosphorylated AXL (p-AXL) and AXL in injured DRGs of SNL rats examined by western blotting and immunofluorescence. Moreover, intrathecal injection of the subthreshold dose of AXL inhibitor TP0903, significantly prolonged the analgesic time of single EA treatment and enhanced the analgesic effect of repeated EA treatments, suggesting a synergic effect of EA and AXL inhibitor. These results indicate that AXL signaling underlies EA analgesia and combination of AXL inhibitor and EA might be a new strategy for clinic analgesia on neuropathic pain. |
| Databáze: | OpenAIRE |
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