Identification and cytogenetic analysis of an abnormal pig chromosome for flow cytometry and sorting
| Autor: | M Hausmann, D Kerboeuf, Christoph Cremer, J. Dölle, Jeannine Boscher, C.P. Popescu |
|---|---|
| Přispěvatelé: | Laboratoire de cytogénétique (UNCEIA/INRA), Institut National de la Recherche Agronomique (INRA), Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Swine Marker chromosome [SDV]Life Sciences [q-bio] Chromosomal translocation Chromosome Disorders Biology General Biochemistry Genetics and Molecular Biology Translocation Genetic 030218 nuclear medicine & medical imaging Flow cytometry Cell Line 03 medical and health sciences 0302 clinical medicine Gene mapping medicine Animals Metaphase ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics Chromosome Aberrations 0303 health sciences medicine.diagnostic_test Cytogenetics Chromosome Chromosome Mapping Karyotype Fibroblasts Flow Cytometry Molecular biology Chromosome Banding [SDV] Life Sciences [q-bio] ANALYSE Karyotyping |
| Zdroj: | Zeitschrift fur Naturforschung B Zeitschrift fur Naturforschung B, Verlag der Zeitschrift Fuer Naturforschung, 1993, 48c, pp.645-653 |
| ISSN: | 0932-0776 |
| Popis: | For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to establish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosom e 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosom es were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosom e 6/15. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|