Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions
| Autor: | Gabriella Pinto, Simonetta Caira, Francesco Addeo, Lina Chianese, Andrea Scaloni, Maria Grazia Calabrese, Maria Adalgisa Nicolai, Sergio Lilla |
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| Přispěvatelé: | Caira, S, Nicolai, MARIA ADALGISA, Lilla, S, Calabrese, Mg, Pinto, Gabriella, Scaloni, A, Chianese, Lina, Addeo, Francesco |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Resolution (mass spectrometry) Buffaloes Hydroxyapatite column 01 natural sciences Analytical Chemistry 03 medical and health sciences Cheese Animals Peptide markers Detection limit g2- and g3-casein Peptide markers Milk Cheese Proteomics Chromatography Chemistry Isoelectric focusing 010401 analytical chemistry Caseins General Medicine Beta2- and Beta3-casein 0104 chemical sciences 030104 developmental biology Milk Water buffalo Cattle Food Science |
| Zdroj: | Food chemistry 230 (2017): 482–490. doi:10.1016/j.foodchem.2017.03.055 info:cnr-pdr/source/autori:Simonetta Caira, Maria Adalgisa Nicolai, Sergio Lilla, Maria Grazia Calabrese, Gabriella Pinto, Andrea Scaloni, Lina Chianese, Francesco Addeo/titolo:Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions/doi:10.1016%2Fj.foodchem.2017.03.055/rivista:Food chemistry/anno:2017/pagina_da:482/pagina_a:490/intervallo_pagine:482–490/volume:230 |
| DOI: | 10.1016/j.foodchem.2017.03.055 |
| Popis: | The European reference method (ERM) recognises the fraudulent addition of bovine (B) milk in water buffalo (WB) milk/dairy products based on concomitant isoelectric focusing (IEF) detection of B Beta2- and Beta3-CN fragments after corresponding plasminolysis. We here used proteomics to characterise false positive results occurring in the ERM as being due to WB Beta-CN(f100-209), which is also formed after plasminolysis of genuine WB milk/dairy products and comigrates in IEF with B Beta2-CN. These ERM limitations were overcome by a dedicated proteomic procedure based on loading of B/WB milk/cheese CN extracts on a hydroxyapatite column, in situ trypsinolysis and elution of B Beta-CN(f1-25)4P and WB Beta-CN(f1-28)4P proteotypic peptides. Based on their similar ionisation properties and resolution in MALDI-TOF-MS, these phosphopeptides were identified as suitable markers for detection of B material in WB milk/dairy products to a detection limit of 0.8% v/v. This proteomic procedure is here proposed as integrative/alternative to the ERM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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