Network analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin
| Autor: | Daniela D. Moré, Wilson Malagó-Jr, B. P. Sollero, Maurício A. Mudadu, Adriana Mércia Guaratini Ibelli, Claudia Cristina Gulias-Gomes, Luiz Lehmann Coutinho, Luciana Correia de Almeida Regitano, Fernando Flores Cardoso |
|---|---|
| Přispěvatelé: | Daniela D. Moré, CPPSE, FERNANDO FLORES CARDOSO, CPPSUL, MAURICIO DE ALVARENGA MUDADU, CNPTIA, WILSON MALAGO JUNIOR, CPPSE, CLAUDIA CRISTINA GULIAS GOMES, CPPSUL, BRUNA PENA SOLLERO, CPPSUL, ADRIANA MERCIA GUARATINI IBELLI, CNPSA, Luiz L. Coutinho, USP, LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
| Rok vydání: | 2019 |
| Předmět: |
Tick infestation
Candidate gene lcsh:QH426-470 lcsh:Biotechnology 030231 tropical medicine Cattle Diseases Tick Plant disease resistance 03 medical and health sciences 0302 clinical medicine lcsh:TP248.13-248.65 parasitic diseases Genetics medicine Animals RNA-Seq Gene Wnt Signaling Pathway 030304 developmental biology Disease Resistance 2. Zero hunger Bovino 0303 health sciences Enrichment analysis biology Gene Expression Profiling Bovine Resistência Genética medicine.disease biology.organism_classification Carrapato Tick Infestations Gene expression profiling lcsh:Genetics Gado Braford Rhipicephalus microplus Cattle Gene expression Resistência SEQUENCIAMENTO GENÉTICO Transcriptome Leukocyte chemotaxis Biotechnology Transcription Factors Research Article Braford |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA BMC Genomics BMC Genomics, Vol 20, Iss 1, Pp 1-20 (2019) |
| Popis: | Background Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were performed to identify genetic pathways and transcription factors (TFs) affecting host resistance. Results Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51 differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs with potential anti-tick functions suggested a role in the development of different levels of resistance within the same breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were only found in TopDEGs from R hosts. Also, these genes indicated the participation of cytokines, such as IL6 and IL22, and the activation of Wingless (WNT)-signaling pathway. A central gene of this pathway, WNT7A, was consistently modulated when hosts were compared. Moreover, the findings based on a genome-wide association study (GWAS) corroborate the prediction of the WNT-signaling pathway as a candidate mechanism of resistance. The regulation of immune response was the most relevant pathway predicted for S hosts. Members of Ap1 and NF-kB families were the most relevant TFs predicted for R and S, respectively. Conclusion This work provides indications of genetic mechanisms presented by Braford cattle with different levels of resistance in response to tick infestation, contributing to the search of candidate genes for tick resistance in bovine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|