Genotypic and phenotypic variation among Lysobacter capsici strains isolated from Rhizoctonia suppressive soils
| Autor: | A.F. Yassin, E.H. Nijhuis, Joeke Postma |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Genotype renaturation rates Molecular Sequence Data Lysobacter Rhizoctonia genus Applied Microbiology and Biotechnology Microbiology DNA Ribosomal dna hybridization Rhizoctonia solani RNA Ribosomal 16S Sequence Homology Nucleic Acid Genetic variation Botany base ratio Cluster Analysis bacteria Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Netherlands Genetic diversity Biointeracties and Plant Health biology sp nov Nucleic Acid Hybridization Sequence Analysis DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA DNA Fingerprinting Bacterial Typing Techniques DNA profiling PRI Biointeractions en Plantgezondheid |
| Zdroj: | Systematic and Applied Microbiology 33 (2010) 4 Systematic and Applied Microbiology, 33(4), 232-235 |
| ISSN: | 1618-0984 0723-2020 |
| Popis: | Four Gram-negative bacterial strains, recovered from clay soils cultivated with different crops in the Netherland, were subjected to a polyphasic taxonomic study in order to clarify their taxonomic status. Comparative analysis of the 16S rRNA gene sequences revealed that they belong to the genus Lysobacter and to be highly related to the type strains of L. antibioticus DSM 2044(T), L. gummosus DSM 6980(T), and L. capsici DSM 19286(T), displaying 99.1-99.3%, 99.2-99.6% and 99.4-100% sequence similarities, respectively, to these species. The results of DNA-DNA hybridization studies unambigiously indicated that the four strains belonged to the species L. capsici. Nevertheless, DNA fingerprinting and phenotypic characterization indicated that there was a considerable diversification and niche differentiation among the strains belonging to L. capsici. The newly identified L. capsici strains strongly inhibit Rhizoctonia solani AG2 and originate from Rhizoctonia-suppressive soils where also populations of L. antibioticus and L. gummosus were present. This is the first report of the presence of combined populations of closely related Lysobacter spp. within agricultural soils. |
| Databáze: | OpenAIRE |
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