Coacervation of Elastin-Like Recombinamer Microgels
| Autor: | Khosrow Rahimi, Dan E. Demco, Radu Fechete, Smriti Singh, José Carlos Rodríguez-Cabello, Martin Möller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Relaxometry
Magic angle Materials science Polymers and Plastics 02 engineering and technology 010402 general chemistry 01 natural sciences Phase Transition Biomimetic Materials Phase (matter) Polymer chemistry Materials Chemistry Spectroscopy Drug Carriers Coacervate Microgeles Organic Chemistry Temperature Flocculation 021001 nanoscience & nanotechnology 0104 chemical sciences Elastin Miniemulsion Chemical engineering Proton NMR Thermodynamics Emulsions 0210 nano-technology Drug carrier Gels Hydrophobic and Hydrophilic Interactions Oligopeptides |
| Zdroj: | UVaDOC. Repositorio Documental de la Universidad de Valladolid instname |
| DOI: | 10.1002/marc.201500457 |
| Popis: | Producción Científica The investigation of the coacervation (self-aggregation) behavior of biomicrogels which can potentially be used as drug carriers is an important topic, because self-aggregation can not only cause loss of activity, but also toxicity and immunogenicity. To study this effect microgels from elastin-like recombinamer are synthesized using miniemulsion technique. The existence of coacervation for such microgels, at different concentrations and temperatures, is studied and proved by cryo-field emission scanning clectron microscopy (cryo-FESEM), cryo-transmission electron microscopy (cryo-TEM), and by a novel 1H high-resolution magic angle sample spinning (HRMAS), nuclear magnetic resonance (NMR) spectroscopy, and relaxometry methods. The findings by 1H HRMAS NMR spectroscopy and relaxometry show simultaneous processes of volume phase temperature transition and coacervation with different sensitivity for hydrophobic and hydrophilic amino acid side-chains in the microgel. The coacervation process is more evidential by the behavior of glycine α-CH2, 1H NMR peak as compared to the proline β-CH2. 2019-12-12 Ministerio de Industria, Economía y Competitividad (proyectos MAT2013-42473-R y MAT2013-41723-R) |
| Databáze: | OpenAIRE |
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