Turnip Mosaic Virus Components Are Released into the Extracellular Space by Vesicles in Infected Leaves
| Autor: | Daniel Garcia Cabanillas, Jean-François Laliberté, Juan Wan, Nooshin Movahed, Hojatollah Vali, Huanquan Zheng |
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| Přispěvatelé: | McGill University = Université McGill [Montréal, Canada], Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Institut National de la Recherche Scientifique [Québec] (INRS), This research was supported by grants from the Natural Sciences and Engineering Research Council (NSERC) of Canada and from le Fonds de Recherche du Québec sur la Nature et les Technologies (FRQNT) to J.-F. L and H.Z., We would like to thank the research group of the Facility for Electron Microscopy Research (FEMR) at McGill University, where we conducted all the experiments of transmission electron microscopy and Focused Ion Beam Systems and Dual Beam TM (FIB) microscopy. We thank Roger Innes (Indiana State University) for providing 35S::GFP -PEN1 and 35S::GmMANI49sYFP transgenic plants |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0106 biological sciences Physiology Viral protein viruses Potyvirus Arabidopsis Nicotiana benthamiana Plant Science Endoplasmic Reticulum Virus Replication medicine.disease_cause 01 natural sciences Viral Proteins 03 medical and health sciences Microscopy Electron Transmission Tobacco Genetics Extracellular medicine Turnip mosaic virus Endomembrane system News and Views 030304 developmental biology 0303 health sciences biology Chemistry Vesicle Multivesicular Bodies food and beverages Immunogold labelling biology.organism_classification 3. Good health Cell biology Plant Leaves [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Viral replication Host-Pathogen Interactions Microscopy Electron Scanning RNA Viral Extracellular Space 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2019, pp.00381.2019. ⟨10.1104/pp.19.00381⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.19.00381 |
| Popis: | International audience; Turnip mosaic virus (TuMV) reorganizes the endomembrane system of the infected cell to generate endoplasmic reticulum-derived motile vesicles containing viral replication complexes. The membrane-associated viral protein 6K2 plays a key role in the formation of these vesicles. Using confocal microscopy, we observed that this viral protein, a marker for viral replication complexes, localized in the extracellular space of infected Nicotiana benthamiana leaves. Previously, we showed that viral RNA is associated with multi-vesicular bodies (MVBs). Here, using transmission electron microscopy, we observed the proliferation of MVBs during infection and their fusion with the plasma membrane that resulted in the release of their intraluminal vesicles in the extracellular space. Immunogold labeling with a monoclonal antibody that recognizes double-stranded RNA indicated that the released vesicles contained viral RNA. Focused ion beam-extreme high-resolution scanning electron microscopy was used to generate a 3D image that showed extracellular vesicles in the cell wall. The presence of TuMV proteins in the extracellular space was confirmed by proteomic analysis of purified extracellular vesicles from Nicotiana benthamiana and Arabidopsis thaliana. Host proteins involved in biotic defense and in inter-organelle vesicular exchange were also detected. The association of extracellular vesicles with viral proteins and RNA emphasizes the implication of the plant extracellular space in viral infection. |
| Databáze: | OpenAIRE |
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