In vitro and in vivo effects of selenium and selenium with vitamin E on platelet functions in diabetic rats relationship to platelet sorbitol and fatty acid distribution
| Autor: | Muriel Bost, Françoise Borson-Chazot, Maryvonne Ciavatti, Michèle Accominotti, Christelle Douillet |
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| Rok vydání: | 1996 |
| Předmět: |
Blood Platelets
Male medicine.medical_specialty Platelet Aggregation Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry chemistry.chemical_element Biochemistry Diabetes Mellitus Experimental Rats Sprague-Dawley Inorganic Chemistry Selenium chemistry.chemical_compound Thrombin In vivo Internal medicine medicine Animals Sorbitol Vitamin E Platelet Selenomethionine Incubation Chromatography High Pressure Liquid chemistry.chemical_classification Fatty Acids Biochemistry (medical) Fatty acid Drug Synergism General Medicine Rats Adenosine Diphosphate Disease Models Animal Endocrinology chemistry Food Fortified Linear Models Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Biological Trace Element Research. 55:263-277 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/bf02785285 |
| Popis: | In vitro 30 min of incubation with selenomethionine (Sm) + vitamin E multiplied by about five platelet selenium (Se) decreased significantly platelet thrombin and ADP-induced aggregation decrease. Four groups of streptozotocin-induced diabetic rats were fed with a supplemented purified diet with an Se-rich yeast (Selenion): DSel, Sm: DSm, Sm alpha-tocopherol: DSmE or unsupplemented diet: D. After 24 wk of supplementation, only a decrease in thrombin-induced aggregation in group DSel compared to DSm and DSmE and D was observed. However, after 24 wk of diet compared to 14 wk, in group D and DSm, a significant increase in thrombin-induced aggregation occurred (p0.0001), whereas a significant decrease in groups DSel and DSmE (p0.0001, p0.03) was noted. After 21 wk of diet, in DSmE, platelet adhesion to fibronectin was significantly decreased compared to group D (p0.05). These changes in DSmE were associated with a significant decrease in platelet sorbitol (p0.02) and a very significant increase in platelet Se (p0.0005). Sm associated with vitamin E would appear more efficient to prevent oxidative damage of diabetic platelet membrane and thus to modulate its hyperactivity. |
| Databáze: | OpenAIRE |
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