RNA-Seq for Bacterial Gene Expression
Autor: | Line Dahl Poulsen, Jeppe Vinther |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Computer science Pipeline (computing) genetic processes RNA-Seq Computational biology Biochemistry DNA sequencing Phosphates 03 medical and health sciences Gene expression natural sciences Bacteria Sequence Analysis RNA Organic Chemistry library construction RNA Gene Expression Regulation Bacterial Bacterial RNA RNA Bacterial 030104 developmental biology ComputingMethodologies_PATTERNRECOGNITION Genes Bacterial RNA Ribosomal next-generation sequencing Analysis tools RNA-seq Multiplex Polymerase Chain Reaction |
Zdroj: | Poulsen, L D & Vinther, J 2018, ' RNA-Seq for Bacterial Gene Expression ', Current Protocols in Nucleic Acid Chemistry, vol. 73, no. 1, e55, pp. 1-12 . https://doi.org/10.1002/cpnc.55 |
DOI: | 10.1002/cpnc.55 |
Popis: | RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-seq experiment is the preparation of sequencing libraries, which is also essential for the quality of the data obtained. Here, we present a straightforward and inexpensive basic protocol for preparation of strand-specific RNA-seq libraries from bacterial RNA as well as a computational pipeline for the data analysis of sequencing reads. The protocol is based on the Illumina platform and allows easy multiplexing of samples and the removal of sequencing reads that are PCR duplicates. © 2018 by John Wiley & Sons, Inc. |
Databáze: | OpenAIRE |
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