Effect of flow and peristaltic mixing on bacterial growth in a gut-like channel
| Autor: | John T. Sauls, Alex Groisman, Igor Segota, Markus Arnoldini, Zhongge Zhang, Chih-yu Yang, Edgar Gutierrez, Jonas Cremer, Terence Hwa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Colony Count 030106 microbiology Population peristalsis Colony Count Microbial Bacterial population bacterial growth Bacterial growth Biology digestive system Models Biological Oral and gastrointestinal Microbiology Diffusion in vitro gut model 03 medical and health sciences Microbial Models Clinical Research Proximal colon education Cancer Peristalsis education.field_of_study reaction-diffusion model Multidisciplinary Bacteria Colonic wall Biological Colo-Rectal Cancer Volumetric flow rate Gastrointestinal Tract reaction–diffusion model 030104 developmental biology Physical Sciences Fluidic channel Biophysics colon microbiota Digestive Diseases Infection Rheology |
| Zdroj: | Proceedings of the National Academy of Science of the United States of America Proceedings of the National Academy of Sciences of the United States of America, vol 113, iss 41 |
| DOI: | 10.1073/pnas.1601306113 |
| Popis: | The ecology of microbes in the gut has been shown to play important roles in the health of the host. To better understand microbial growth and population dynamics in the proximal colon, the primary region of bacterial growth in the gut, we built and applied a fluidic channel that we call the “minigut.” This is a channel with an array of membrane valves along its length, which allows mimicking active contractions of the colonic wall. Repeated contraction is shown to be crucial in maintaining a steady-state bacterial population in the device despite strong flow along the channel that would otherwise cause bacterial washout. Depending on the flow rate and the frequency of contractions, the bacterial density profile exhibits varying spatial dependencies. For a synthetic cross-feeding community, the species abundance ratio is also strongly affected by mixing and flow along the length of the device. Complex mixing dynamics due to contractions is described well by an effective diffusion term. Bacterial dynamics is captured by a simple reaction–diffusion model without adjustable parameters. Our results suggest that flow and mixing play a major role in shaping the microbiota of the colon. |
| Databáze: | OpenAIRE |
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