DNA Protection against Oxidative Damage Using the Hydroalcoholic Extract ofGarcinia mangostanaand Alpha-Mangostin

Autor: Martin Brendel, João Antonio Pêgas Henriques, Alanna Cibelle Fernandes Pereira, Cristina Pungartnik, Fabrício Rios-Santos, Temenouga N. Guecheva, Rúbens Prince dos Santos Alves, Ronaldo Carvalho-Silva
Rok vydání: 2016
Předmět:
Zdroj: Evidence-based Complementary and Alternative Medicine : eCAM
Evidence-Based Complementary and Alternative Medicine, Vol 2016 (2016)
ISSN: 1741-4288
1741-427X
Popis: Garcinia mangostana,popularly known as “mangosteen fruit,” originates from Southeast Asia and came to Brazil about 80 years ago where it mainly grows in the states of Pará and Bahia. Although mangosteen or its extracts have been used for ages in Asian folk medicine, data on its potential genotoxicity is missing. We, therefore, evaluated genotoxicity/mutagenicity of hydroethanolic mangosteen extract [HEGM, 10 to 640 μg/mL] in established test assays (Comet assay, micronucleus test, andSalmonella/microsome test). In the Comet assay, HEGM-exposed human leukocytes showed no DNA damage. No significant HEGM-induced mutation in TA98 and TA100 strains ofSalmonella typhimurium(with or without metabolic activation) was observed and HEGM-exposed human lymphocytes had no increase of micronuclei. However, HEGM suggested exposure concentration-dependent antigenotoxic potential in leukocytes and antioxidant potential in the yeastSaccharomyces cerevisiae. HEGM preloading effectively protected against H2O2-induced DNA damage in leukocytes (Comet assay). Preloading of yeast with HEGM for up to 4 h significantly protected the cells from lethality of chronic H2O2-exposure, as expressed in better survival. Absence of genotoxicity and demonstration of an antigenotoxic and antioxidant potential suggest that HEGM or some substances contained in it may hold promise for pharmaceutical or nutraceutical application.
Databáze: OpenAIRE
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