Sickness Behavior Score Is Associated with Neuroinflammation and Late Behavioral Changes in Polymicrobial Sepsis Animal Model
| Autor: | Mariana Pereira de Souza Goldim, Nivaldo Ferreira, Maria Eduarda Fileti, Amanda Della Giustina, Franciane Bobinski, Fabricia Petronilho, Bruna Hoffmann de Oliveira, Khiany Mathias, Jucélia Jeremias Fortunato, Aloir de Oliveira Junior, João Quevedo, Juliete Palandi, Graciela Freitas Zarbato, Jaqueline S. Generoso, Raquel Jaconi De Carli, Naiana da Rosa, Daniel Martins, Juliana de Oliveira, Leandro Garbossa, Felipe Dal-Pizzol, Tatiana Barichello, Andriele Vieira |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty medicine.medical_treatment Immunology medicine.disease_cause Gastroenterology Sepsis 03 medical and health sciences chemistry.chemical_compound Eating 0302 clinical medicine Corticosterone Laparotomy Internal medicine medicine Immunology and Allergy Animals Rats Wistar Sickness behavior Depression (differential diagnoses) Illness Behavior Inflammation business.industry Coinfection medicine.disease Rheumatology Cognitive test Rats Disease Models Animal Oxidative Stress 030104 developmental biology chemistry 030220 oncology & carcinogenesis Inflammation Mediators business Oxidative stress Locomotion |
| Zdroj: | Inflammation. 43(3) |
| ISSN: | 1573-2576 |
| Popis: | The use of reliable scores is a constant development in critical illness. According to Sepsis-3 consensus, the use of Sequential Organ Failure Assessment (SOFA) score of 2 or more is associated with a higher mortality of sepsis patients. In experimental research, due murine animal model limitations, the use of a score systems can be an alternative to assess sepsis severity. In this work, we suggest a sickness behavior score (SBS) that uses physiological variables to assess sepsis severity and mortality. Animals were evaluated daily by the presence of six indicators of sickness behavior: temperature alteration, preference of water/sucrose, liquid intake, food intake, body weight, and movimentation. Male adult Wistar rats were evaluated daily after sepsis induction by cecal ligation and puncture (CLP) or laparotomy only (sham) for determination of SBS. Oxidative stress, IL-6, and HPA axis markers (corticosterone and adrenal gland weight) were evaluated 24 h after CLP to determine the correlation with the acute SBS and neuroinflammation. Also, BDNF and four cognitive behavioral tests were correlated with the chronic SBS, i.e., sum of 8 days after surgery. In result, septic rats presented higher SBS than sham animals. Sepsis severity markers were associated with acute and chronic SBS. Also, SBS was negative correlated with the cognitive tests. In conclusion, SBS shows to be reliable score to predict sepsis severity and mortality. The use of score system provides the analysis of global sickness behavior, beyond evaluation of each parameter individually. |
| Databáze: | OpenAIRE |
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