Inducibility of various cytochrome P450 isozymes by phenobarbital and some other xenobiotics in Drosophila melanogaster
| Autor: | Vladimir S. Spiegelman, Serge Y. Fuchs, Gennady A. Belitsky |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cytochrome
7-Alkoxycoumarin O-Dealkylase Biochemistry Isozyme chemistry.chemical_compound Cytochrome P-450 Enzyme System Drosophilidae medicine Animals Inducer Enzyme inducer Pharmacology biology Cytochrome P450 biology.organism_classification Isoenzymes Drosophila melanogaster chemistry Enzyme Induction Phenobarbital biology.protein Aryl Hydrocarbon Hydroxylases Xenobiotic medicine.drug |
| Zdroj: | Biochemical pharmacology. 47(10) |
| ISSN: | 0006-2952 |
| Popis: | The inducibility of cytochrome P450 isozymes has been investigated in the Drosophila melanogaster insecticide susceptible (Oregon R) and insecticide resistant (91R) strains. Both the level and induction kinetics of 7-ethoxycoumarine O -deethylase activity were stimulated by phenobarbital (PB) to a lower extent than that of aryl hydrocarbon hydroxylase in the Oregon R strain. The basal level of the cytochrome P450-linked activities in insecticide resistant flies was higher than that noted in susceptible ones. However, treatment with PB has increased levels of 7-ethoxycoumarine O -deethylase and aryl hydrocarbon hydroxylase activities more in susceptible flies than in resistant ones. in contrast to PB, the polycyclic aromatic hydrocarbon benzo[ a ]pyrene induced both activities in 91R flies to a greater extent than in Oregon R ones. The potent PB-like inducer in mice but not in rats 1.4-bis[2-(dichloropyridyloxy)]-benzene failed to induce the cytochrome P450 system in D. melanogaster , when triphenyldioxane (PB-like inducer in rats but not in mice) markedly affected this system in a PB-like manner. The SDS-PAGE followed by immunoblotting analysis using monoclonal antibodies 13-2e and 8-1d have shown that the level of the 56,000 and 54,000 Da insecticide resistance-related forms has increased in the susceptible strain by PB and some other PB like inducers. The relationship between these isozymes appearance and 7-ethoxycoumarine O -deethylase activity has been discussed. |
| Databáze: | OpenAIRE |
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