Decreased expression of p57KIP2 mRNA in human bladder cancer
Autor: | Wolfgang A. Schulz, Mototsugu Oya |
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Rok vydání: | 2000 |
Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty RNA Untranslated medicine.medical_treatment Urinary Bladder LOH analysis Loss of Heterozygosity Muscle Proteins Biology Loss of heterozygosity Cystectomy chromosome 11p15.5 Insulin-Like Growth Factor II Gene expression Tumor Cells Cultured medicine Carcinoma Humans RNA Messenger Urothelium IGF-II Cyclin-Dependent Kinase Inhibitor p57 quantitative RT-PCR Aged Mucous Membrane Bladder cancer Urinary bladder H19 Reverse Transcriptase Polymerase Chain Reaction Chromosomes Human Pair 11 Nuclear Proteins Cancer Regular Article Middle Aged medicine.disease female genital diseases and pregnancy complications medicine.anatomical_structure Urinary Bladder Neoplasms Oncology Cancer research Female RNA Long Noncoding |
Zdroj: | British Journal of Cancer |
ISSN: | 1532-1827 0007-0920 |
Popis: | To identify targets of genetic and epigenetic alterations on chromosome 11p15.5 in human bladder cancer, expression of the imprinted KIP2, IGF2 and H19 genes was studied by quantitative RT-PCR in 24 paired samples of urothelial carcinomas and morphologically normal mucosa obtained by cystectomy, and in bladder carcinoma cell lines. The most frequent alteration in tumour tissue was decreased expression of KIP2 identified in 9/24 (37%) specimens. Decreased IGF2 and H19 mRNA levels were found in five (21%) and three (13%) tumours, respectively. One tumour each overexpressed IGF2 and H19. Loss of H19 expression was only found associated with loss of KIP2 expression, whereas decreased expression of IGF2 mRNA occurred independently. Almost all bladder carcinoma cell lines showed significant changes in the expression of at least one gene with diminished expression of KIP2 mRNA as the most frequent alteration. IGF2 mRNA levels were diminished in several lines, but increased in others. The KIP2 gene could be an important target of genetic and epigenetic alterations in bladder cancer affecting the maternal chromosome 11p15.5. However, reminiscent of the situation in Wilms’ tumours, expression of the IGF2 gene on the paternal chromosome can also be disturbed in bladder cancers. © 2000 Cancer Research Campaign |
Databáze: | OpenAIRE |
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