Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

Autor: M. R. S. Balarim, D. P. Netto, A. A. Alfieri, Nanci do Nascimento, P. L. Bicudo, Simone Biagio Chiacchio
Přispěvatelé: Universidade Estadual de Londrina (UEL), Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP)
Rok vydání: 2004
Předmět:
Zdroj: SciELO
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 10, Iss 1, Pp 34-52 (2004)
Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 10, Issue: 1, Pages: 34-52, Published: 2004
Journal of Venomous Animals and Toxins including Tropical Diseases v.10 n.1 2004
The Journal of venomous animals and toxins including tropical diseases
ISSN: 1678-9199
DOI: 10.1590/s1678-91992004000100004
Popis: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:43:31Z No. of bitstreams: 1 S1678-91992004000100004.pdf: 137622 bytes, checksum: 17ef8356e1a35a2d868c33c6d6e7324f (MD5) Made available in DSpace on 2013-08-22T18:43:31Z (GMT). No. of bitstreams: 1 S1678-91992004000100004.pdf: 137622 bytes, checksum: 17ef8356e1a35a2d868c33c6d6e7324f (MD5) Previous issue date: 2004-01-01 Made available in DSpace on 2013-09-30T19:31:11Z (GMT). No. of bitstreams: 2 S1678-91992004000100004.pdf: 137622 bytes, checksum: 17ef8356e1a35a2d868c33c6d6e7324f (MD5) S1678-91992004000100004.pdf.txt: 35019 bytes, checksum: 0789758e9211f9526a070c68ddf05f43 (MD5) Previous issue date: 2004-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:22Z No. of bitstreams: 2 S1678-91992004000100004.pdf: 137622 bytes, checksum: 17ef8356e1a35a2d868c33c6d6e7324f (MD5) S1678-91992004000100004.pdf.txt: 35019 bytes, checksum: 0789758e9211f9526a070c68ddf05f43 (MD5) Made available in DSpace on 2014-05-20T13:39:22Z (GMT). No. of bitstreams: 2 S1678-91992004000100004.pdf: 137622 bytes, checksum: 17ef8356e1a35a2d868c33c6d6e7324f (MD5) S1678-91992004000100004.pdf.txt: 35019 bytes, checksum: 0789758e9211f9526a070c68ddf05f43 (MD5) Previous issue date: 2004-01-01 Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity. Universidade Estadual de Londrina (UEL) Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo Instituto de Pesquisas Energéticas e Nucleares Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia
Databáze: OpenAIRE
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