Localization of the two major allergens in rye-grass pollen using specific monoclonal antibodies and quantitative analysis of immunogold labelling
| Autor: | Robert Bruce Knox, Mohan Singh, Philip E. Taylor, I. A. Staff |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Tissue Fixation
Starch medicine.drug_class Biology medicine.disease_cause Monoclonal antibody chemistry.chemical_compound Cytosol Antigen Pollen Lolium medicine Paraformaldehyde Plant Proteins Binding Sites Chromatography Antibodies Monoclonal food and beverages Cell Biology Immunogold labelling Allergens Antigens Plant Immunohistochemistry Molecular biology chemistry Glutaraldehyde Anatomy Quantitative analysis (chemistry) |
| Zdroj: | The Histochemical Journal. 26:392-401 |
| ISSN: | 1573-6865 0018-2214 |
| DOI: | 10.1007/bf00160051 |
| Popis: | The intracellular localization of the two major allergens, Lol p I and Lol p IX, in rye-grass anthers was examined using monoclonal antibodies FMCA1 (specific for Lol p I) and FMCA7 (specific for Lol p IX) with immunocytochemical techniques and quantitative analysis. A newly developed anhydrous fixation technique in a mixture of glutaraldehyde, paraformaldehyde and 2,2-dimethoxypropane followed by embedding in LR Gold resin resulted in both improved infiltration of pollen grains compared with existing techniques and the localization of these water-soluble antigens in their original sites compared with diffusion artefacts following aqueous methods. After anhydrous fixation, Lol p I was predominantly located in the electron-opaque regions of the cytosol of the vegetative cell of the tricellular pollen grains (24 counts microns-2), whereas Lol p IX was detected mainly within starch granules (16 counts microns-2). For both Lol p I and Lol p IX, similar labelling was detected in the cells of the endothecium and middle layer (18 counts microns-2), but none was found in the tapetal cells or orbicules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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