Cardinium symbiosis as a potential confounder of mtDNA based phylogeographic inference in Culicoides imicola (Diptera: Ceratopogonidae), a vector of veterinary viruses

Autor: Gregory D. D. Hurst, Gert J. Venter, Stefanos Siozios, Claire Garros, Matthew Baylis, Jack Pilgrim
Přispěvatelé: University of Liverpool, NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, University of Pretoria [South Africa], Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This work was supported by a BBSRC Doctoral Training Partnership studentship (BB/M011186/1) awarded to JP and a Marie Curie Individual Fellowship (H2020-MSCA-IF-2014) grant awarded to SS.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
Linkage disequilibrium
Culicoides imicola
[SDV]Life Sciences [q-bio]
Flux de gènes
L73 - Maladies des animaux
Cytochrome c oxydase
Ceratopogonidae
01 natural sciences
Gene flow
South Africa
Phylogeny
Symbiote
0303 health sciences
education.field_of_study
biology
Mediterranean Region
Culicoides
Bacterial Infections
Phylogeography
Infectious Diseases
Cardinium
Gene Flow
Population
Short Report
010603 evolutionary biology
DNA
Mitochondrial
lcsh:Infectious and parasitic diseases
COI
03 medical and health sciences
Animals
lcsh:RC109-216
Horses
education
Symbiosis
030304 developmental biology
Endosymbiont
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Bacteroidetes
Cytochrome c oxidase subunit I
Haplotype
fungi
Correction
Sequence Analysis
DNA
biology.organism_classification
Endophyte
Insect Vectors
Evolutionary biology
Parasitology
ADN mitochondrial
Zdroj: Parasites & Vectors
Parasites & Vectors, 2021, 14 (1), ⟨10.1186/s13071-020-04568-3⟩
Parasites & Vectors, Vol 14, Iss 1, Pp 1-5 (2021)
Parasites and Vectors
ISSN: 1756-3305
DOI: 10.1186/s13071-020-04568-3⟩
Popis: Background Culicoides imicola (Diptera: Ceratopogonidae) is an important Afrotropical and Palearctic vector of disease, transmitting viruses of animal health and economic significance including African horse sickness and bluetongue viruses. Maternally inherited symbiotic bacteria (endosymbionts) of arthropods can alter the frequency of COI (cytochrome c oxidase subunit I) mitochondrial haplotypes (mitotypes) in a population, masking the true patterns of host movement and gene flow. Thus, this study aimed to assess the mtDNA structure of C. imicola in relation to infection with Candidatus Cardinum hertigii (Bacteroides), a common endosymbiont of Culicoides spp. Methods Using haplotype network analysis, COI Sanger sequences from Cardinium-infected and -uninfected C. imicola individuals were first compared in a population from South Africa. The network was then extended to include mitotypes from a geographic range where Cardinium infection has previously been investigated. Results The mitotype network of the South African population demonstrated the presence of two broad mitotype groups. All Cardinium-infected specimens fell into one group (Fisher’s exact test, P = 0.00071) demonstrating a linkage disequilibrium between endosymbiont and mitochondria. Furthermore, by extending this haplotype network to include other C. imicola populations from the Mediterranean basin, we revealed mitotype variation between the Eastern and Western Mediterranean basins (EMB and WMB) mirrored Cardinium-infection heterogeneity. Conclusions These observations suggest that the linkage disequilibrium of Cardinium and mitochondria reflects endosymbiont gene flow within the Mediterranean basin but may not assist in elucidating host gene flow. Subsequently, we urge caution on the single usage of the COI marker to determine population structure and movement in C. imicola and instead suggest the complementary utilisation of additional molecular markers. Graphical Abstract
Databáze: OpenAIRE
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