HPLC-PDA-MS and NMR Characterization of a Hydroalcoholic Extract of Citrus aurantium L. var. amara Peel with Antiedematogenic Activity
| Autor: | Dulce María Sánchez, Anna Lisa Piccinelli, Teresa Mencherini, Rita Patrizia Aquino, Luca Campone, Milagros García Mesa, Luca Rastrelli |
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| Přispěvatelé: | Mencherini, T, Campone, L, Piccinelli, A, Garcia Mesa, M, Sanchez, D, Aquino, R, Rastrelli, L |
| Rok vydání: | 2012 |
| Předmět: |
Male
Citrus Magnetic Resonance Spectroscopy Citrus aurantium var. amara peel sour orange HPLC-PDA-MS flavonoids coumarins platelet aggregation antiedematogenic activity coumarin Flavones Mass Spectrometry Nobiletin chemistry.chemical_compound Neoeriocitrin Animals Edema Humans flavonoid Rats Wistar Naringin Chromatography High Pressure Liquid chemistry.chemical_classification Neohesperidin Chromatography Plant Extracts General Chemistry Diosmetin Rats Rhoifolin chemistry Fruit General Agricultural and Biological Sciences Flavanone |
| Zdroj: | Journal of Agricultural and Food Chemistry. 61:1686-1693 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf302815t |
| Popis: | The phytochemical profile of a hydroalcoholic extract of Citrus aurantium var. amara L. peel, used as herbal medicine, was characterized by HPLC-PDA-MS. Two di-C-glycosyl flavones (vincenin II and diosmetin 6,8-di-C-glucoside), a series of flavones (luteolin 7-O-neohesperidoside, rhoifolin, and neodiosmin), and flavanone (neoeriocitrin, naringin, and neohesperidin) 7-O-neohesperidosides and two methoxyflavones (nobiletin and tangeretin), commonly present in Citrus, were identified. Furthermore, brutieridin and melitidin, two 3-hydroxy-3-methylglutaryl flavanone glycosides, were also characterized along with rhoifolin 4′-glucoside and three coumarins (8,3′-β-d- glucopyranosyloxy-2′-hydroxy-3′-methylbutyl-7-methoxycoumarin, merazin hydrate, and isomerazin). A preparative isolation procedure followed by NMR spectroscopy confirmed the proposed structures of the major flavonoids and identified the coumarins. The phenolic content was found to be 14.8 mg mL -1, and naringin and neohesperidin were the compounds present in the highest concentration (3.6 and 2.6 mg mL-1). The extract of C. aurantium peel inhibited significantly (p < 0.05) both histamine- and dextran-induced edema in rats in a concentration-dependent manner (IC 50 = 119.6 and 118.3 mg kg-1, respectively), providing evidence for the therapeutic use of C. aurantium var. amara peel. © 2012 American Chemical Society |
| Databáze: | OpenAIRE |
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