A proteomic approach to studying plant response to crenate broomrape (Orobanche crenata) in pea (Pisum sativum)

Autor: Nardjis Amiour, Eliane Dumas-Gaudot, M. Ángeles Castillejo, Diego Rubiales, Jesús Jorrín
Přispěvatelé: Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), ProdInra, Migration
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Proteomics
0106 biological sciences
Silver Staining
Genotype
Parasitic plant
Nitrogen assimilation
Gene Expression
Plant Science
Horticulture
Orobanche crenata
Peptide Mapping
Plant Roots
01 natural sciences
Biochemistry
Fructokinase
Pisum
03 medical and health sciences
Sativum
Glutamine synthetase
Electrophoresis
Gel
Two-Dimensional
Databases
Protein
Molecular Biology
[SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
ComputingMilieux_MISCELLANEOUS
Plant Proteins
030304 developmental biology
Pathogenesis-related protein
2. Zero hunger
0303 health sciences
biology
Orobanche
Peas
General Medicine
biology.organism_classification
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Electrophoresis
Polyacrylamide Gel
010606 plant biology & botany
Zdroj: Phytochemistry
Phytochemistry, Elsevier, 2004, 65, pp.1817-1828
ISSN: 0031-9422
Popis: Crenate broomrape ( Orobanche crenata ) is a parasitic plant that threatens legume production in Mediterranean areas. Pea ( Pisum sativum ) is severely affected, and only moderate levels of genetic resistance have so far been identified. In the present work we selected the most resistant accession available (Ps 624) and compared it with a susceptible (Messire) cultivar. Experiments were performed by using pot and Petri dish bioassays, showing little differences in the percentage of broomrape seed germination induced by both genotypes, but a significant hamper in the number of successfully installed tubercles and their developmental stage in the Ps 624 compared to Messire. The protein profile of healthy and infected P. sativum root tissue were analysed by two-dimensional electrophoresis. Approximately 500 individual protein spots could be detected on silver stained gels. At least 22 different protein spots differentiated control, non-infected, Messire and Ps 624 accessions. Some of them were identified by MALDI-TOF mass spectrometry and database searching as cysteine proteinase, β-1,3-glucanase, endochitinase, profucosidase, and ABA-responsive protein. Both qualitative and quantitative differences have been found among infected and non-infected root extracts. Thus, in the infected susceptible Messire genotype 34 spots were decreased, one increased and three newly detected, while in Ps 624, 15 spots were increased, three decreased and one newly detected. In response to the inoculation, proteins that correspond to enzymes of the carbohydrate metabolism (fructokinase, fructose-bisphosphate aldolase), nitrogen metabolism (ferredoxin-NADP reductase) and mitochondrial electronic chain transport (alternative oxidase 2) decreased in the susceptible check, while proteins that correspond to enzymes of the nitrogen assimilation pathway (glutamine synthetase) or typical pathogen defence, PR proteins, including β-1,3-glucanase and peroxidases, increased in Ps 624. Results are discussed in terms of changes in the carbohydrate and nitrogen metabolism an induction of defence proteins in response to broomrape parasitism.
Databáze: OpenAIRE
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