FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer
| Autor: | Aaron Chang, Mark E. Mummert, Ignacy Gryczynski, Rahul Chib, Sangram Raut, Ryan Rich, Zygmunt Gryczynski, Rafal Fudala |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty animal structures Urinary Bladder Urology Hyaluronoglucosaminidase Pharmaceutical Science Urine Article Fluorescence Rhodamine chemistry.chemical_compound Prostate cancer Biomarkers Tumor Fluorescence Resonance Energy Transfer medicine Humans Hyaluronic Acid Fluorescein Urinary bladder Bladder cancer Rhodamines Chemistry Prostatic Neoplasms medicine.disease medicine.anatomical_structure Förster resonance energy transfer Urinary Bladder Neoplasms Biochemistry Biomarker (medicine) Biotechnology |
| Zdroj: | Current Pharmaceutical Biotechnology. 14:470-474 |
| ISSN: | 1389-2010 |
| Popis: | Elevated hyaluronidase levels are found in the urine of bladder and prostate cancer patients. Therefore, HA-ase is regarded as an important biomarker for the detection of these cancers. In this report, we use a FRET based ratiometric sensing approach to detect the level of HA-ase in synthetic urine. For this, we have used a HA-FRET probe (hyaluronan) labeled with fluorescein as a donor and rhodamine as an acceptor. We monitor the digestion of our HA-FRET probe with different concentrations of HA-ase in synthetic urine via fluorescence emission. The extent to which FRET is released depends on the concentration of HA-ase. Our fluorescence intensity results are also supported with time resolved fluorescence decay data. This assay can be used to develop a non-invasive technique for the detection of bladder and/or prostate cancer progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|