Criticality of plasma membrane lipids reflects activation state of macrophage cells
| Autor: | Fiona J. E. Morgan, Raphaelle Taub, Eugenia Cammarota, Chiara Soriani, Clare E. Bryant, Sarah L. Veatch, Pietro Cicuta, Jiro Sakai |
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| Přispěvatelé: | Morgan, Fiona [0000-0003-0583-7996], Bryant, Clare [0000-0002-2924-0038], Cicuta, Pietro [0000-0002-9193-8496], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
liquid–liquid phase separation
Lipopolysaccharide Membrane lipids Biomedical Engineering Biophysics Bioengineering Inflammation Biochemistry Biomaterials 03 medical and health sciences chemistry.chemical_compound Membrane Lipids 0302 clinical medicine Immune system Cell surface receptor macrophage activation medicine Interleukin 4 030304 developmental biology 0303 health sciences Innate immune system Macrophages Cell Membrane critical lipidomics Membrane Proteins Life Sciences–Physics interface plasma membrane composition Cell biology Protein Transport Membrane chemistry Membrane protein medicine.symptom 030217 neurology & neurosurgery Biotechnology Research Article |
| Zdroj: | Journal of the Royal Society Interface |
| DOI: | 10.1101/680157 |
| Popis: | Signalling is of particular importance in immune cells, and upstream in the signalling pathway many membrane receptors are functional only as complexes, co-locating with particular lipid species. Work over the last 15 years has shown that plasma membrane lipid composition is close to a critical point of phase separation, with evidence that cells adapt their composition in ways that alter the proximity to this thermodynamic point. Macrophage cells are a key component of the innate immune system, are responsive to infections and regulate the local state of inflammation. We investigate changes in the plasma membrane’s proximity to the critical point as a response to stimulation by various pro- and anti-inflammatory agents. Pro-inflammatory (interferonγ, Kdo 2-Lipid A, lipopolysaccharide) perturbations induce an increase in the transition temperature of giant plasma membrane vesicles; anti-inflammatory interleukin 4 has the opposite effect. These changes recapitulate complex plasma membrane composition changes, and are consistent with lipid criticality playing a master regulatory role: being closer to critical conditions increases membrane protein activity. |
| Databáze: | OpenAIRE |
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