Evaluation of the In Vitro Damage Caused by Lipid Factors on Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence
Autor: | Carley Cooper, Guiting Lin, Robert Gelfand, Nestor F. Gonzalez-Cadavid, Alec Ohanian, William Brent DeCastro, Istvan Kovanecz, Sheila Sharifzad, Tom F. Lue |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
endocrine system diseases Urinary Incontinence Stress medicine.medical_treatment Palmitic Acid Type 2 diabetes Myostatin wound closure lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine stem cell damage lcsh:QH301-705.5 Cells Cultured Spectroscopy microRNA biology Stem Cells apoptosis free fatty acids General Medicine Stem-cell therapy Computer Science Applications myostatin 030220 oncology & carcinogenesis fat infiltration Biomarker (medicine) Female Stem cell medicine.medical_specialty muscle-derived stem cells Article Catalysis Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Animals Obesity Physical and Theoretical Chemistry Molecular Biology business.industry Cholesterol dyslipidemia Organic Chemistry cholesterol nutritional and metabolic diseases medicine.disease In vitro Rats Rats Zucker Disease Models Animal 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 Diabetes Mellitus Type 2 chemistry Apoptosis biology.protein business |
Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 14 International Journal of Molecular Sciences, Vol 21, Iss 5045, p 5045 (2020) |
ISSN: | 1422-0067 |
Popis: | Human stem cell therapy for type 2 diabetes/obesity (T2D/O) complications is performedwith stem cell autografts, exposed to the noxious T2D/O milieu, often with suboptimal results.We showed in the Obese Zucker (OZ) rat model of T2D/O that when their muscle-derived stemcells (MDSC) were from long-term T2D/O male rats, their repair ecacy for erectile dysfunctionwas impaired and were imprinted with abnormal gene- and miR-global transcriptional signatures(GTS). The damage was reproduced in vitro by short-term exposure of normal MDSC to dyslipidemicserum, causing altered miR-GTS, fat infiltration, apoptosis, impaired scratch healing, and myostatinoverexpression. Similar in vitro alterations occurred with their normal counterparts (ZF4-SC) fromthe T2D/O rat model for female stress urinary incontinence, and with ZL4-SC from non-T2D/O leanfemale rats. In the current work we studied the in vitro eects of cholesterol and Na palmitate aslipid factors on ZF4-SC and ZL4-SC. A damage partially resembling the one caused by the femaledyslipidemic serum was found, but diering between both lipid factors, so that each one appears tocontribute specifically to the stem cell damaging eects of dyslipidemic serum in vitro and T2D/Oin vivo, irrespective of gender. These results also confirm the miR-GTS biomarker value forMDSC damage. |
Databáze: | OpenAIRE |
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