Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells
| Autor: | Toshiyuki Takehara, Takeshi Teramura, Chiaki Hamanishi, Koichi Nakagawa, Yuta Onodera, Kanji Fukuda |
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| Rok vydání: | 2012 |
| Předmět: |
Homeobox protein NANOG
Cellular differentiation Induced Pluripotent Stem Cells Biophysics Biology Mechanotransduction Cellular Biochemistry SOX2 Tensile Strength Humans Phosphorylation Induced pluripotent stem cell Molecular Biology Cells Cultured Homeodomain Proteins rho-Associated Kinases Induced stem cells SOXB1 Transcription Factors Nanog Homeobox Protein Cell Biology Embryonic stem cell Cell biology Endothelial stem cell Gene Expression Regulation Stem cell Octamer Transcription Factor-3 Proto-Oncogene Proteins c-akt |
| Zdroj: | Biochemical and Biophysical Research Communications. 417:836-841 |
| ISSN: | 0006-291X |
| Popis: | Mechanical stimulation has been shown to regulate the proliferation and differentiation of stem cells. However, the effects of the mechanical stress on the stemness or related molecular mechanisms have not been well determined. Pluripotent stem cells such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are used as good materials for cell transplantation therapy and research of mammalian development, since they can self-renew infinitely and differentiate into various cell lineages. Here we demonstrated that the mechanical stimulation to human iPS cells altered alignment of actin fibers and expressions of the pluripotent related genes Nanog, POU5f1 and Sox2. In the mechanically stimulated iPS cells, small GTPase Rho was activated and interestingly, AKT phosphorylation was decreased. Inhibition of Rho-associated kinase ROCK recovered the AKT phosphorylation and the gene expressions. These results clearly suggested that the Rho/ROCK is a potent primary effector of mechanical stress in the pluripotent stem cells and it participates to pluripotency-related signaling cascades as an upper stream regulator. |
| Databáze: | OpenAIRE |
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