Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O
| Autor: | Tamara Martinović, Marija Begić, Dajana Gašo-Sokač, Hrvoje Pavlović, Djuro Josic, Martina Šrajer Gajdošik, Olga Shevchuk, Uroš Andjelković, James Clifton |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Pyridoxal 030309 nutrition & dietetics Bacterial Toxins Down-Regulation Bacterial growth medicine.disease_cause Foodborne pathogens Listeriolysin O Mechanism of action Proteome changes Growth inhibition Biomarker candidate Fast diagnosis of listeriosis Virulence factor Cell wall Hemolysin Proteins 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Listeria monocytogenes Tandem Mass Spectrometry medicine Heat-Shock Proteins 0303 health sciences biology Gene Expression Regulation Bacterial 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Membrane protein chemistry Biochemistry Bacteria Chromatography Liquid Food Science |
| Zdroj: | Food Research International. 131:108951 |
| ISSN: | 0963-9969 |
| Popis: | Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down- regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed. |
| Databáze: | OpenAIRE |
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