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Po-sa plant possesses many useful biological activities which have been the reason for the selection of this plant for the present paper. The present paper is concerned with detection of acute toxicity, antioxidant and cholesterol lowering activities of Po-sa (leaves and stem).In acute toxicity tests, watery and 80% EtOH extracts of both leaves and stem were free from acute toxic effects up to the dose of 12g/ kg body weight. Antioxidant activity of Po-sa (leaves and stem) extracts was performed by using spectroscopic DPPH assay method. The IC50 values of EtOAc, EtOH and watery extracts of leaves were 2.39μg/mL, 2.11μg/mL and 2.15μg/mL respectively. . The IC50 values of EtOAc, EtOH and watery extracts of stem were 2.94μg/mL, 1.21μg/mL and 1.20μg/mL respectively. The EtOH and watery extracts of stem showed more potent radical scavenging activity than those of leaves. Cholesterol lowering activity of crude extracts of leaves and stem were also studied by Zlatkis, Zak and Boyle method on rats model. The cholesterol levels were determined after orally administrated rats with 600 mg/kg/day of plant extracts for 10 days. It indicated that watery extract of stem has the ability to reduce bad cholesterol (TC, TG and VLDL) and it also could effectively increase the good blood cholesterol (HDL) level. Therefore, Po-sa stem has good antioxidant and cholesterol lowering activities. It can be used as a natural source of antioxidants to prevent the progression of many diseases. |
