Differential expression of Vitamin D associated genes in the aorta of coronary artery disease patients with and without rheumatoid arthritis
| Autor: | Arne Yndestad, Pål Aukrust, Torstein Lyberg, Stein Erik Rynning, Ole Kristoffer Olstad, Jon Elling Whist, Ingvild Oma, Sven M. Almdahl, Ida Grunnan Fostad, Morten W. Fagerland, Ivana Hollan, J.K. Andersen, Øyvind Molberg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
rheumatoid arthritis 0301 basic medicine Microarrays NSAIDs vascular pathology Gene Expression Organic chemistry lcsh:Medicine Arthritis Cell Cycle Proteins Vascular Medicine Arthritis Rheumatoid Coronary artery disease 0302 clinical medicine Medicine and Health Sciences Coronary Heart Disease Vitamin D lcsh:Science Aorta Analgesics Multidisciplinary Nuclear Proteins Drugs Vitamins Physical sciences Chemistry Bioassays and Physiological Analysis Rheumatoid arthritis language Female coronary artery disease Research Article medicine.medical_specialty Patients Immunology ascending aorta Cardiology Norwegian Research and Analysis Methods Autoimmune Diseases Chemical compounds 03 medical and health sciences Rheumatology Internal medicine medicine.artery Organic compounds Genetics medicine Vitamin D and neurology Humans Nuclear Receptor Co-Repressor 1 RNA Messenger Aged Pharmacology 030203 arthritis & rheumatology Aryldialkylphosphatase business.industry Public health lcsh:R Biology and Life Sciences DNA Microarray Analysis medicine.disease Pain management language.human_language Health Care immune system Cross-Sectional Studies 030104 developmental biology Clinical Immunology lcsh:Q Clinical Medicine atherosclerosis business Rheumatism |
| Zdroj: | 13:e0202346 PLoS ONE PLoS ONE, Vol 13, Iss 8, p e0202346 (2018) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Vitamin D has an important role in the immune system, and has been linked to rheumatoid arthritis (RA) and coronary artery disease (CAD). The exact mechanisms by which vitamin D is involved in these processes are still unclear. Therefore, we wanted to search for differences in expression of genes involved in the vitamin D receptor (VDR) activation pathway and genes that are known to alter upon vitamin D stimulation, in the aortic adventitia of CAD patients with and without RA. METHODS: Affymetrix microarray was used to determine gene expression profile in surgical specimens from the adventitia of the ascending aorta of CAD patients with RA (n = 8) and without RA (n = 8) from the Feiring Heart Biopsy Study. RESULTS: We identified three vitamin D associated genes that were differentially expressed between RA and non-RA patients: Growth arrest and DNA-damage-inducible protein 45 alpha (GADD45A) (FC = 1.47; p = 0.006), Nuclear Receptor Co-repressor 1 (NCOR1) (FC = 1,21; p = 0.005) and paraoxonases 2 (PON2) (FC = -1.37; p = 0.01). High expression of GADD45A in RA tissues was confirmed by real-time qRT-PCR. GADD45A expression correlated with plasma levels of 1,25(OH)2D3 (rs = 0.69; p = 0.003). CONCLUSIONS: Microarray analyses revealed higher expression of GADD45A and NCOR1; and lower expression of PON2 in the aortic adventitia of RA than non-RA patients. Further studies are needed to elucidate if and how GADD45A, NCOR1 and PON2 are involved in the development of accelerated atherosclerosis in RA. In theory, some of these factors might have proatherogenic effects whereas others might reflect an underlying vascular pathology promoting atherogenesis (such as vascular stress). This study was supported by the Innlandet Hospital Trust, South-Eastern Norway Regional Health Authority, the Norwegian Women’s Public Health Association, the Norwegian Rheumatism Association, and the Norwegian Association for People with Heart and Lung Diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|