Size Control and Fluorescence Labeling of Polydopamine Melanin-Mimetic Nanoparticles for Intracellular Imaging
| Autor: | King Hang Aaron Lau, Devang Amin, Phillip B. Messersmith, Caroline Sugnaux |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Fluorophore Biomedical Engineering Nanoparticle Nanotechnology Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry lcsh:Technology Fluorescence spectroscopy Article Biomaterials chemistry.chemical_compound Dynamic light scattering Zeta potential QD lcsh:T nanoparticle Photothermal therapy catechol 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences melanin chemistry PEGylation Molecular Medicine dopamine 0210 nano-technology Biotechnology |
| Zdroj: | Biomimetics (Basel, Switzerland) Biomimetics; Volume 2; Issue 3; Pages: 17 Biomimetics, vol 2, iss 3 Biomimetics, Vol 2, Iss 3, p 17 (2017) Biomimetics (Basel, Switzerland), vol 2, iss 3 |
| ISSN: | 2313-7673 |
| Popis: | As synthetic analogs of the natural pigment melanin, polydopamine nanoparticles (NPs) are under active investigation as non-toxic anticancer photothermal agents and as free radical scavenging therapeutics. By analogy to the widely adopted polydopamine coatings, polydopamine NPs offer the potential for facile aqueous synthesis and incorporation of (bio)functional groups under mild temperature and pH conditions. However, clear procedures for the convenient and reproducible control of critical NP properties such as particle diameter, surface charge, and loading with functional molecules have yet to be established. In this work, we have synthesized polydopamine-based melanin-mimetic nanoparticles (MMNPs) with finely controlled diameters spanning ≈25 to 120 nm and report on the pH-dependence of zeta potential, methodologies for PEGylation, and the incorporation of fluorescent organic molecules. A comprehensive suite of complementary techniques, including dynamic light scattering (DLS), cryogenic transmission electron microscopy (cryo-TEM), X-ray photoelectron spectroscopy (XPS), zeta-potential, ultraviolet–visible (UV–Vis) absorption and fluorescence spectroscopy, and confocal microscopy, was used to characterize the MMNPs and their properties. Our PEGylated MMNPs are highly stable in both phosphate-buffered saline (PBS) and in cell culture media and exhibit no cytotoxicity up to at least 100 µg mL−1 concentrations. We also show that a post-functionalization methodology for fluorophore loading is especially suitable for producing MMNPs with stable fluorescence and significantly narrower emission profiles than previous reports, suggesting they will be useful for multimodal cell imaging. Our results pave the way towards biomedical imaging and possibly drug delivery applications, as well as fundamental studies of MMNP size and surface chemistry dependent cellular interactions. |
| Databáze: | OpenAIRE |
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