Upregulated Expression of Intestinal Antimicrobial Peptide HD5 Associated with Renal Function in IgA Nephropathy

Autor: Dianchun Shi, Lanping Jiang, Zhong Zhong, Xiaoyan Li, Jinjin Fan, Shaozhen Feng
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
alpha-Defensins
Medicine (General)
medicine.medical_specialty
Article Subject
Clinical Biochemistry
030232 urology & nephrology
Renal function
urologic and male genital diseases
Gastroenterology
Nephropathy
Young Adult
03 medical and health sciences
Sex Factors
R5-920
0302 clinical medicine
Internal medicine
Genetics
medicine
Humans
Molecular Biology
Survival analysis
Kidney
medicine.diagnostic_test
business.industry
Biochemistry (medical)
Case-control study
Glomerulonephritis
IGA
Glomerulonephritis
General Medicine
Middle Aged
medicine.disease
Survival Analysis
Up-Regulation
030104 developmental biology
medicine.anatomical_structure
Case-Control Studies
Multivariate Analysis
Regression Analysis
Female
Renal biopsy
business
Glomerular Filtration Rate
Research Article
Kidney disease
Zdroj: Disease Markers
Disease Markers, Vol 2020 (2020)
ISSN: 1875-8630
0278-0240
DOI: 10.1155/2020/2078279
Popis: Purpose. It was reported that gut-kidney axis may play an important role in IgA nephropathy (IgAN). Previous five GWASs of different populations for IgAN have discovered several genes related to intestinal immunity, including DEFA gene. However, the roles of the encoded proteins of DEFA5/6 which were called intestinal antimicrobial peptides HD5 and HD6 were not clear in kidney disease, such as IgAN. The purpose of this study was to clarify the association of HD5 and HD6 with IgAN. Methods. We measured HD5 and HD6 in serum, urine, and kidney of IgAN patients and normal controls by ELISA, Western blot, and immunofluorescence. The association of HD5 or HD6 levels with clinical and pathologic phenotypes was analyzed. Results. Serum levels of HD5 and HD6 were significantly higher in IgAN patients than those in normal controls. Baseline serum HD5 levels were significantly associated with eGFR (P=0.002) and tubular atrophy/interstitial fibrosis (P=0.004) by stepwise multivariate regression analysis. Compared to the patients with serum HD5 below the median level, patients with elevated serum HD5 above the median level had a significantly worse renal outcome (log-rank test, P=0.009) by Kaplan-Meier analysis. A Cox regression model showed that serum HD5 was an independent prognostic factor (HR=1.239, P=0.029) after adjusting for the well-known predictors of outcome in IgAN patients. In renal biopsies of IgAN patients, HD5 was significantly expressed in the damaged proximal tubules, while no immunoreactive HD6 was found. Interestingly, the serum HD6 level of IgAN patients was significantly associated with gender. Conclusions. In IgAN patients, an elevated serum HD5 level at the time of renal biopsy was associated with poor renal outcomes. HD5 rather than HD6 was probably associated with renal function of IgAN patients.
Databáze: OpenAIRE
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