β-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase
| Autor: | Paula Sánchez, Beatriz Baragaña, Shirley M. Roberts, Dolores González-Pacanowska, Keith S. Wilson, Reto Brun, Marcel Kaiser, Ian H. Gilbert, Orla McCarthy, Nils Gunnar Johansson, Cristina Bosch-Navarrete, Jean L. Whittingham, Xiao-Xiong Zhou |
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| Rok vydání: | 2010 |
| Předmět: |
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Molecular Stereochemistry Clinical Biochemistry Plasmodium falciparum Pharmaceutical Science Biochemistry chemistry.chemical_compound Antimalarials Structure-Activity Relationship Drug Discovery Structure–activity relationship Humans Enzyme Inhibitors Pyrophosphatases Molecular Biology chemistry.chemical_classification biology Organic Chemistry Active site Uracil biology.organism_classification Deoxyuridine Uridine Enzyme chemistry biology.protein Molecular Medicine DNA |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1464-3391 |
| Popis: | We report a series of β-branched acyclic tritylated deoxyuridine analogues as inhibitors of Plasmodium falciparum deoxyuridine-5′- triphosphate nucleotidohydrolase (PfdUTPase), an enzyme involved in nucleotide metabolism that acts as first line of defence against uracil incorporation into DNA. Compounds were assayed against both PfdUTPase and intact parasites showing a correlation between enzyme inhibition and cellular assays. β-Branched acyclic uridine analogues described here showed equal or slightly better potency and selectivity compared with previously reported analogues. The best inhibitor gave a Ki of 0.5 μM against PfdUTPase with selectivity greater than 200-fold compared to the corresponding human enzyme and sub-micromolar growth inhibition of P. falciparum (EC50 0.6 μM). A crystal structure of the complex of PfdUTPase with one of the inhibitors shows that this acyclic derivative binds to the active site in a similar manner to that previously reported for a tritylated cyclic deoxyuridine derivative. © 2011 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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