DNA repair gene OGG1 polymorphism and its relation with oxidative DNA damage in patients with Alzheimer’s disease
| Autor: | Yildiz Dincer, Serap Yavuzer, Gökhan Erkol, Melda Bozluolcay, Tuba Mutlu, Mehmet Güven, Çağlayan Akkaya |
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| Přispěvatelé: | Tıp Fakültesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male DNA Repair DNA repair Biology Polymorphism Single Nucleotide law.invention DNA Glycosylases 03 medical and health sciences 0302 clinical medicine law Alzheimer Disease Humans Alzheimer’s Disease Genotyping Polymerase chain reaction Aged chemistry.chemical_classification Aged 80 and over General Neuroscience 8-Hydroxydeoxyguanosine Base excision repair Middle Aged OGG1 Polymorphism Molecular biology Comet assay Oxidative Stress 030104 developmental biology Enzyme chemistry DNA glycosylase Case-Control Studies Female Oxidative DNA Damage Restriction fragment length polymorphism 030217 neurology & neurosurgery DNA Damage |
| Popis: | There is considerable evidence that oxidative DNA damage is increased, DNA repair capacity is decreased in patients with Alzheimer's disease. Base excision repair is the major pathway in removal of oxidative DNA damage. 8-oxo-deoxyguanosine DNA glycosylase 1 (OGG1) is the enzyme which is involved in the first step of this repair process. Alterations in DNA repair capacity may be related with polymorphisms in DNA repair genes. In order to investigate the effect of OGG1 Ser326Cys polymorphism on oxidative DNA damage level, OGG1 genotyping was performed, basal and oxidative DNA damage in lymphocytes and 8-OHdG level in plasma were examined in patients with Alzheimer's disease. Basal and oxidative DNA damage and 8-OHdG level were measured by OGG1-modified comet assay and enzyme-linked immunoassay, respectively. OGG1 genotyping was performed by polymerase chain reaction- restriction fragment length polymorphism assay. Basal and oxidative DNA damage and plasma 8-OHdG levels were found to be higher in the Alzheimer's disease group than those in the control group (P 0.001). In the Alzheimer's disease group, the levels of oxidative DNA damage was higher in the patients having OGG1 (Ser326Cys + Cys326Cys) genotype than those in the patients having OGG1 Ser326Ser genotype. It was concluded that oxidative DNA damage is increased in patients with Alzheimer's disease and OGG1 Ser326Cys polymorphism may be responsible for this increase. |
| Databáze: | OpenAIRE |
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