In Vitro Selection of Probiotics, Prebiotics, and Antioxidants to Develop an Innovative Synbiotic (NatuREN G) and Testing Its Effect in Reducing Uremic Toxins in Fecal Batches from CKD Patients
| Autor: | Mirco Vacca, Marcello Salvatore Lenucci, Loreto Gesualdo, Maria De Angelis, Fabio Minervini, Angelo Santino, Giuseppe Celano, Flavia la Forgia, Sergio Fontana, Aurelia Scarano, Giuseppe Dalfino |
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| Přispěvatelé: | Vacca, M., Celano, G., Lenucci, M. S., Fontana, S., la Forgia, F. M., Minervini, F., Scarano, A., Santino, A., Dalfino, G., Gesualdo, L., De Angelis, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antioxidant fecal batches QH301-705.5 medicine.medical_treatment Inulin Prebiotic 030204 cardiovascular system & hematology Probiotic Microbiology Article law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine fluids and secretions law Virology Chronic kidney disease medicine microbiota Food science Biology (General) Bifidobacterium biology Chemistry Microbiota Fecal batche synbiotic food and beverages biology.organism_classification Bifidobacterium animalis Synbiotic 030104 developmental biology antioxidants Proanthocyanidin probiotics Quercetin prebiotics chronic kidney disease |
| Zdroj: | Microorganisms Microorganisms, Vol 9, Iss 1316, p 1316 (2021) Volume 9 Issue 6 |
| ISSN: | 2076-2607 |
| Popis: | We aimed to develop an innovative synbiotic formulation for use in reducing dysbiosis, uremic toxins (e.g., p-cresol and indoxyl sulfate), and, consequently, the pathognomonic features of patients with chronic kidney disease (CKD). Twenty-five probiotic strains, belonging to lactobacilli and Bifidobacterium, were tested for their ability to grow in co-culture with different vegetable (pomegranate, tomato, and grapes) sources of antioxidants and prebiotics (inulin, fructo-oligosaccharides, and β-glucans). Probiotics were selected based on the acidification rates and viable cell counts. Inulin and fructo-oligosaccharides reported the best prebiotic activity, while a pomegranate seed extract was initially chosen as antioxidant source. The investigation was also conducted in fecal batches from healthy and CKD subjects, on which metabolomic analyses (profiling volatile organic compounds and total free amino acids) were conducted. Two out of twenty-five probiotics were finally selected. After the stability tests, the selective innovative synbiotic formulation (named NatuREN G) comprised Bifidobacterium animalis BLC1, Lacticaseibacillus casei LC4P1, fructo-oligosaccharides, inulin, quercetin, resveratrol, and proanthocyanidins. Finally, NatuREN G was evaluated on fecal batches collected from CKD in which modified the viable cell densities of some cultivable bacterial patterns, increased the concentration of acetic acid and decane, while reduced the concentration of nonanoic acid, dimethyl trisulfide, and indoxyl sulfate. |
| Databáze: | OpenAIRE |
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