Toxicological assessment of PEGylated single-walled carbon nanotubes in early developing zebrafish
| Autor: | Adelina P. Santos, Ana Paula Horn, Lidiane Dal Bosco, Sangram K. Sahoo, Carla O. F. Gonçalves, Gisele Eva Bruch, Carolina S. Peixoto, Daniela M. Barros, Felipe A. Girardi, Cristiano Fantini, Marcos F. Cordeiro |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Time Factors DNA damage Embryonic Development 02 engineering and technology Carbon nanotube Polyethylene glycol Toxicology law.invention Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound law PEG ratio Animals Zebrafish Pharmacology Dose-Response Relationship Drug Nanotubes Carbon 021001 nanoscience & nanotechnology Molecular Weight Oxidative Stress 030104 developmental biology chemistry Nanotoxicology Larva Toxicity PEGylation Biophysics Surface modification 0210 nano-technology Reactive Oxygen Species DNA Damage |
| Zdroj: | Toxicology and applied pharmacology. 347 |
| ISSN: | 1096-0333 |
| Popis: | Functionalization of single-walled carbon nanotubes (SWCNT) with polyethylene glycol (PEG) is among the most promising strategies to avoid SWCNT aggregation in aqueous media, improving its interactions with biological systems. However, the best molecular PEG weight and functionalization strategy remain under investigation. In this work we assessed the toxicological effects of SWCNT functionalized with PEG at 600 Da in zebrafish embryos. Embryos were exposed to SWCNT at 0.01, 0.1 and 1 mg/L from 3 to 96 h post-fertilization (hpf). At the highest concentration, SWCNT led to toxic effects at several endpoints, including mortality, delayed hatching, malformations, reduced body length, increased ROS production and DNA damage. Even with these effects, SWCNT could not be detected within the bodily tissues of the larvae. Our results give evidence that the tested PEGylation approach was unsuitable to avoid SWCNT aggregation in aqueous media, and that SWCNT can induce toxicity even without being absorbed by the organism by obstructing the chorion pores. |
| Databáze: | OpenAIRE |
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