Single‐Sperm Typing
| Autor: | K. Schmitt, Joanna Szyda, Sigbjørn Lien, Norman Arnheim, Esther P. Leeflang, Lin Zhang, Rene S. Hubert |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
Male Sequence analysis Genetics Medical Single-nucleotide polymorphism Cell Separation Biology Polymerase Chain Reaction Primer extension law.invention law Genetics Humans Typing Genetics (clinical) Polymerase chain reaction Sepharose Haplotype Sequence Analysis DNA Flow Cytometry Spermatozoa Sperm Genetic Techniques Haplotypes Genetic marker |
| Zdroj: | Current Protocols in Human Genetics |
| ISSN: | 1934-8258 1934-8266 |
| DOI: | 10.1002/0471142905.hg0106s32 |
| Popis: | This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed. |
| Databáze: | OpenAIRE |
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