Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif
| Autor: | Wei Xia, Adam Davis, Peng Li, Christopher J. Burrell, Feng Feng, Julie-anne Lake, Jill Carr |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytidine deaminase activity
Gene Products vif Transcription Genetic Immunoprecipitation viruses Ubiquitin-Protein Ligases Immunology Blotting Western Molecular Sequence Data Replication Biology Virus Replication Microbiology Virus Cell Line Virology vif Gene Products Human Immunodeficiency Virus Humans Amino Acid Sequence RNA Messenger APOBEC3G Zinc finger Infectivity Viral Reverse Transcription Intracellular Signaling Peptides and Proteins virus diseases Zinc Fingers biochemical phenomena metabolism and nutrition Precipitin Tests Viral replication Gene Expression Regulation Insect Science DNA Viral HIV-1 Carrier Proteins Gene Deletion Protein Binding |
| Zdroj: | Flinders University PURE |
| ISSN: | 0022-538X |
| Popis: | Virion infectivity factor (Vif) protein of human immunodeficiency virus type 1 (HIV-1) is essential for the productive infection of primary human CD4 T lymphocytes and macrophages. Vif overcomes the HIV-inhibitory effects of cellular factor APOBEC3G, which has cytidine deaminase activity. We previously reported the isolation of a Vif-interacting ring finger protein, Triad 3, from a human leukocyte cDNA library, using the yeast two-hybrid system. The full-length cellular protein homologue of Triad 3 has been recently identified as the zinc finger protein inhibiting NF-κB (ZIN). Sequence analysis indicates that Triad 3 protein contains all four major ring-like motifs of ZIN. We report here that ZIN binds to purified Vif in vitro and that Triad 3/ZIN interacts with HIV-1 Vif in transfected human 293T cells, as demonstrated by coimmunoprecipitation. To test the biological relevance of this interaction, we produced infectious HIV-1 NL4.3 in the presence or absence of cotransfected ZIN. HIV-1 NL4.3 virus stocks produced in the presence of exogenously expressed ZIN were twofold less infectious in a single-cycle infectivity assay than virus produced in the absence of exogenous ZIN. It was further shown that cells infected with HIV NL4.3 virus stocks produced in the presence of exogenously expressed ZIN were impaired in viral DNA synthesis by twofold. The impairment in viral reverse transcription and the reduction in single-cycle viral infectivity were both shown to be dependent on the presence of Vif in the virus producer cells. The possible mechanisms by which ZIN interferes with the early events of HIV-1 replication are discussed. |
| Databáze: | OpenAIRE |
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