Sulforaphane alleviates retinal ganglion cell death and inflammation by suppressing NLRP3 inflammasome activation in a rat model of retinal ischemia/reperfusion injury
| Autor: | Lei Gong, Xiaoning Cao, Weiguo Li, Yuerong Gong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Retinal Ganglion Cells
Programmed cell death Inflammasomes Immunology sulforaphane Apoptosis Pharmacology Neuroprotection Retina Proinflammatory cytokine Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine inflammasome Isothiocyanates NLR Family Pyrin Domain-Containing 3 Protein medicine Animals Immunology and Allergy retinal ganglion cell Letter to the Editor Inflammation Cell Death Microglia Inflammasome Retinal medicine.disease Rats Disease Models Animal Neuroprotective Agents medicine.anatomical_structure Retinal ganglion cell chemistry Reperfusion Injury Sulfoxides 030221 ophthalmology & optometry Female sense organs Reperfusion injury 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | International Journal of Immunopathology and Pharmacology |
| ISSN: | 2058-7384 |
| DOI: | 10.1177/2058738419861777 |
| Popis: | This study aims to study the potentials of sulforaphane (SFN) against retinal ischemia/reperfusion (I/R) injury. A rat retinal I/R injury method was established. Retinal thickness change and retinal ganglion cell (RGC) death were determined using hematoxylin and eosin (H&E) staining and Fluoro-Gold (FG) labeling. The inflammatory cytokines production and microglia activation were evaluated by using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and enzyme-linked immunosorbent assay (ELISA). Knockdown NLRP3 was performed, and the according changes of retinal RGCs were assessed. SFN administration significantly inhibited I/R and caused retinal thickness change and prevented RGCs death in retinal I/R model. SFN suppressed inflammatory cytokines production, microglia activation, and inflammasome activation. In accordance, NLRP3 knockdown presented the similar inhibitory effect on I/R rats. This study demonstrates that SFN prevents RGCs death and acts as a potent neuroprotective modulator in retinal I/R injury, which may be associated with inhibition of the NLRP3 inflammasome activation. |
| Databáze: | OpenAIRE |
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