Comparison of Pharmacokinetics and Anti-Pulmonary Fibrosis-Related Effects of Sulforaphane and Sulforaphane N-acetylcysteine
| Autor: | Han-Joo Maeng, Jin-Ha Yoon, Yu Chul Kim, Sung Hwan Jeong, Xiang Fei, Seung-Yong Seo, Eun Suk Son |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Metabolite Pharmaceutical Science sulforaphane Pharmacology Article metabolic stability Acetylcysteine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacy and materia medica Pharmacokinetics Oral administration In vivo medicine Prodrug sulforaphane N-acetylcysteine RS1-441 030104 developmental biology chemistry anti-pulmonary fibrosis effect 030220 oncology & carcinogenesis Isothiocyanate pharmacokinetics medicine.drug Sulforaphane |
| Zdroj: | Pharmaceutics, Vol 13, Iss 958, p 958 (2021) Pharmaceutics Volume 13 Issue 7 |
| ISSN: | 1999-4923 |
| Popis: | Sulforaphane (SFN), belonging to the isothiocyanate family, has received attention owing to its beneficial activities, including chemopreventive and antifibrotic effects. As sulforaphane N-acetylcysteine (SFN-NAC), a major sulforaphane metabolite, has presented similar pharmacological activities to those of SFN, it is crucial to simultaneously analyze the pharmacokinetics and activities of SFN and SFN-NAC, to comprehensively elucidate the efficacy of SFN-containing products. Accordingly, the anti-pulmonary fibrotic effects of SFN and SFN-NAC were assessed, with simultaneous evaluation of permeability, metabolic stability, and in vivo pharmacokinetics. Both SFN and SFN-NAC decreased the levels of transforming growth factor-β1-induced fibronectin, alpha-smooth muscle actin, and collagen, which are major mediators of fibrosis, in MRC-5 fibroblast cells. Regarding pharmacokinetics, SFN and SFN-NAC were metabolically unstable, especially in the plasma. SFN-NAC degraded considerably faster than SFN in plasma, with SFN being formed from SFN-NAC. In rats, SFN and SFN-NAC showed a similar clearance when administered intravenously however, SFN showed markedly superior absorption when administered orally. Although the plasma SFN-NAC concentration was low owing to poor absorption following oral administration, SFN-NAC was converted to SFN in vivo, as in plasma. Collectively, these data suggest that SFN-NAC could benefit a prodrug formulation strategy, possibly avoiding the gastrointestinal side effects of SFN, and with improved SFN-NAC absorption. |
| Databáze: | OpenAIRE |
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