Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D
| Autor: | Norman K. Fry, Jens Ø. Duus, Ian C. Skovsted, Sofie Slott, Georgia Kapatai, Christian Kjeldsen, Pernille L. Elverdal, Carmen L. Sheppard |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Magnetic Resonance Spectroscopy Hybrid serotype 030106 microbiology medicine.disease_cause Serogroup Biochemistry Analytical Chemistry Microbiology 03 medical and health sciences NMR spectroscopy Antigen Bacterial Proteins Streptococcus pneumoniae medicine Animals Whole genome sequence Serotyping Bacterial Capsules Whole genome sequencing chemistry.chemical_classification Antiserum Molecular Structure Immune Sera Structure elucidation Organic Chemistry Glycosyltransferase Gene Polysaccharides Bacterial Glycosyltransferases General Medicine Sequence Analysis DNA Amino acid chemistry Mutation Quellung reaction Rabbits Genome Bacterial |
| Zdroj: | Kjeldsen, C, Slott, S, Elverdal, P L, Sheppard, C L, Kapatai, G, Fry, N K, Skovsted, I C & Duus, J Ø 2018, ' Discovery and description of a new serogroup 7 Streptococcus pneumoniae serotype, 7D, and structural analysis of 7C and 7D ', Carbohydrate Research, vol. 463, pp. 24-31 . https://doi.org/10.1016/j.carres.2018.04.011 |
| Popis: | Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D. |
| Databáze: | OpenAIRE |
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