ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis

Autor: Dominique Giorgi, Francis Poulat, Ariane Abrieu, Julien Espeut, Sylvie Rouquier, Jean-Michel Saffin, Magali Venoux, Claude Prigent
Přispěvatelé: Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS), Grants from Association pour la Recherche sur le Cancer and Ligue Nationale contre le Cancer (to S.R.) and Équipe Labellisée LNCC (to C.P.)., Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS)-IFR122-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Prigent, Claude
Rok vydání: 2005
Předmět:
MESH: Cytokinesis
MESH: Sequence Analysis
DNA
Microtubule-associated protein
Blotting
Western
Molecular Sequence Data
MESH: RNA Interference
Oligonucleotides
Aurora B kinase
MESH: Microscopy
Fluorescence
Spindle Apparatus
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Polo-like kinase
MESH: Base Sequence
Biology
03 medical and health sciences
0302 clinical medicine
MESH: Oligonucleotides
Microtubule
Humans
MESH: Blotting
Western
MESH: Cloning
Molecular
Cloning
Molecular
MESH: Spindle Apparatus
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Mitosis
Cells
Cultured
Cytokinesis
Glutathione Transferase
030304 developmental biology
MESH: Glutathione Transferase
0303 health sciences
MESH: Humans
MESH: Molecular Sequence Data
Multidisciplinary
Base Sequence
Sequence Analysis
DNA
Biological Sciences
3. Good health
Cell biology
Spindle apparatus
MESH: Microtubule-Associated Proteins
Microscopy
Fluorescence
Mitotic exit
030220 oncology & carcinogenesis
RNA Interference
Microtubule-Associated Proteins
MESH: Cells
Cultured
Zdroj: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (32), pp.11302-7. ⟨10.1073/pnas.0500964102⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2005, 102 (32), pp.11302-7. ⟨10.1073/pnas.0500964102⟩
ISSN: 1091-6490
0027-8424
DOI: 10.1073/pnas.0500964102
Popis: We have identified a unique human microtubule-associated protein (MAP) named ASAP for AS ter- A ssociated P rotein. ASAP localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis and directly binds to purified microtubules by its COOH-terminal domain. Overexpression of ASAP induces profound bundling of cytoplasmic microtubules in interphase cells and aberrant monopolar spindles in mitosis. Depletion of ASAP by RNA interference results in severe mitotic defects: it provokes aberrant mitotic spindle, delays mitotic progression, and leads to defective cytokinesis or cell death. These results suggest a crucial role for ASAP in the organization of the bipolar mitotic spindle, mitosis progression, and cytokinesis and define ASAP as a key factor for proper spindle assembly.
Databáze: OpenAIRE
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