Cytidine triphosphate synthase activity and mRNA expression in normal human blood cells
| Autor: | Peter Vreken, A. B. P. Van Kuilenburg, A. H. van Gennip, Arnauld Verschuur, P.A. Voûte, E.J. Muller |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Blood Platelets
Cytidine triphosphate Erythrocytes Anabolism viruses Clinical Biochemistry Biochemistry Monocytes chemistry.chemical_compound Humans Platelet Nucleotide Centrifugation Carbon-Nitrogen Ligases heterocyclic compounds Lymphocytes RNA Messenger Molecular Biology Whole blood chemistry.chemical_classification ATP synthase biology Chemistry Blotting Northern Molecular biology Enzyme Activation enzymes and coenzymes (carbohydrates) Enzyme biology.protein Granulocytes |
| Zdroj: | Biological chemistry, 380(1), 41-46. Walter de Gruyter GmbH |
| ISSN: | 1431-6730 |
| Popis: | Cytidine triphosphate (CTP) synthase is one of the key enzymes in pyrimidine nucleotide anabolic pathways. The activity of this enzyme is elevated in various malignancies including acute lymphocytic leukemia (ALL). In this study we investigated the activity of CTP synthase in various human blood cells isolated from healthy volunteers by density centrifugation and elutriation centrifugation. We also investigated the mRNA expression of CTP synthase in lymphocytes and monocytes. The highest activity of CTP synthase was found in thrombocytes (6.48 nmol CTP × mg−1× h−1), followed by that of monocytes (2.23), lymphocytes (1.69), granulocytes (0.52) and erythrocytes (0.42). The activity of CTP synthase in whole blood samples was at an intermediate level (1.27). The mRNA expression of CTP synthase in monocytes was comparable to that observed in lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|