Nuclear E-Cadherin Acetylation Promotes Colorectal Tumorigenesis via Enhancing β-Catenin Activity
| Autor: | Yongxu Zhao, Jianxia Chen, Yue Qin, Li-Jun Luo, Shuang Li, Feng Liu, Zhenling Cui, Peng Zhang, Tao Yu, Nan Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Carcinogenesis medicine.disease_cause SIRT2 03 medical and health sciences 0302 clinical medicine Antigens CD Cell Line Tumor medicine Humans Molecular Biology beta Catenin Cell Nucleus Chemistry Acetylation Cadherins HCT116 Cells Actin cytoskeleton HEK293 Cells 030104 developmental biology Oncology Tumor progression 030220 oncology & carcinogenesis Catenin Cancer research Signal transduction Colorectal Neoplasms Nuclear localization sequence |
| Zdroj: | Molecular Cancer Research. 17:655-665 |
| ISSN: | 1557-3125 1541-7786 |
| DOI: | 10.1158/1541-7786.mcr-18-0637 |
| Popis: | The E-cadherin/β-catenin signaling pathway plays a critical role in the maintenance of epithelial architecture and regulation of tumor progression. Normally, E-cadherin locates on the cell surface with its cytosolic domain linking to the actin cytoskeleton through interaction with catenins. Although the nuclear localization of E-cadherin has been frequently observed in various types of cancers, little is known regarding the functional consequences of its nuclear translocation. Here, we showed that in colorectal cancer samples and cell lines, E-cadherin localized in the nucleus; and the nuclear localization was mediated through protein interaction with CTNND1. In the nucleus, E-cadherin was acetylated by CREB-binding protein at Lysine870 and Lysine871 in its β-catenin–binding domain, and the acetylation can be reversed by SIRT2. Acetylation of nuclear E-cadherin attenuated its interaction with β-catenin, which therefore released β-catenin from the complex, resulting in increased expression of its downstream genes and accelerated tumor growth and migration. Further study showed that acetylation level of nuclear E-cadherin had high prognostic significance in clinical colorectal samples. Taken together, our findings reveal a novel mechanism of tumor progression through posttranslational modification of E-cadherin, which may serve as a potential drug target of tumor therapy. Implications: This finding that acetylation of nuclear E-cadherin regulates β-catenin activity expands our understanding of the acetylation of E-cadherin promotes colorectal cancer cell growth and suggests novel therapeutic approaches of targeting acetylation in tumors. |
| Databáze: | OpenAIRE |
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