Application of an indirect immunofluorescence test for detection of Mycoplasma pneumoniae in respiratory exudates
| Autor: | Yasuhiro Kanemasa, Yoshikazu Hirai, Toyohisa Masayoshi, Junji Shiode |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
Mycoplasma pneumoniae Fluorescent Antibody Technique Cross Reactions medicine.disease_cause Sensitivity and Specificity Microbiology Antigen Antibody Specificity Pneumonia Mycoplasma medicine Humans Antiserum Antigens Bacterial biology Exudates and Transudates biology.organism_classification Antibodies Bacterial Mucus Titer Evaluation Studies as Topic Polyclonal antibodies biology.protein Mollicutes Antibody Research Article |
| Zdroj: | Journal of Clinical Microbiology. 29:2007-2012 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.29.9.2007-2012.1991 |
| Popis: | We prepared polyclonal antibody specific to Mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antigen in respiratory exudates. The antibody did not cross-react with normal human serum or with respiratory exudates from 10 healthy persons. Cross-reactivity of the antibody with species of mycoplasmas other than M. genitalium was fully diminished when absorbed with horse serum and yeast extract, components of the culture medium. Though the absorbed antibody cross-reacted with M. genitalium, the titer was significantly lower than when tested against M. pneumoniae. Two types of antigen-specific fluorescence were observed in clinical specimens: one is large or small fluorescent granular aggregates found in mucus, and the other is fine fluorescent particles diffused on the entire surface of small epithelial cells. Throat smears from 49 patients with serologically confirmed M. pneumoniae infections were examined by our indirect immunofluorescence method. Positive results were obtained in 42 cases, many of which were positive before a rise in serum antibody titer could be demonstrated, indicating that the method is useful for a preliminary diagnosis at an early stage of the infection. |
| Databáze: | OpenAIRE |
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