It takes two for chronic wounds to heal: dispersing bacterial biofilm and modulating inflammation with dual action plasma coatings
| Autor: | Rahul Madathiparambil Visalakshan, Dung Thuy Thi Tran, Thomas D. Michl, Barbora Ivanovská, Krasimir Vasilev, Hannah Frederike Kuckling, Laura E. González García, Aigerim Zhalgasbaikyzy |
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| Přispěvatelé: | Michl, Thomas Danny, Tran, Dung Thuy Thi, Kuckling, Hannah Frederike, Zhalgasbaikyzy, Aigerim, Ivanovská, Barbora, González García, Laura Elena, Madathiparambil Visalakshan, Rahul, Vasilev, Krasimir |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
medicine.medical_treatment Population Inflammation 02 engineering and technology coatings engineering.material 03 medical and health sciences Coating Staphylococcus epidermidis Dual action medicine education staphylococcus epidermidis 030304 developmental biology 0303 health sciences education.field_of_study biology Atomic force microscopy Chemistry Biofilm cell signalling x-ray photoelectron spectroscopy General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Cytokine engineering Biophysics biofilm formation bacterial biofilm medicine.symptom 0210 nano-technology |
| Popis: | Chronic wounds are affecting increasingly larger portions of the general population and their treatment has essentially remained unchanged for the past century. This lack of progress is due to the complex problem that chronic wounds are simultaneously infected and inflamed. Both aspects need to be addressed together to achieve a better healing outcome. Hence, we hereby demonstrate that the stable nitroxide radical (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) can be plasma polymerized into smooth coatings (TEMPOpp), as seen via atomic force microscopy, X-ray photoelectron spectroscopy and ellipsometry. Upon contact with water, these coatings leach nitroxides into aqueous supernatant, as measured via EPR. We then exploited the known cell-signalling qualities of TEMPO to change the cellular behaviour of bacteria and human cells that come into contact with the surfaces. Specifically, the TEMPOpp coatings not only suppressed biofilm formation of the opportunistic bacterium Staphylococcus epidermidis but also dispersed already formed biofilm in a dose-dependent manner; a crucial aspect in treating chronic wounds that contain bacterial biofilm. Thus the coatings' microbiological efficacy correlated with their thickness and the thickest coating was the most efficient. Furthermore, this dose-dependent effect was mirrored in significant cytokine reduction of activated THP-1 macrophages for the four cytokines TNF-α, IL-1β, IL-6 and IP-10. At the same time, the THP-1 cells retained their ability to adhere and colonize the surfaces, as verified via SEM imaging. Thus, summarily, we have exploited the unique qualities of plasma polymerized TEMPO coatings in targeting both infection and inflammation simultaneously; demonstrating a novel alternative to how chronic wounds could be treated in the future. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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